Description

2,5-Dioxopyrrolidin-1-Yl 5-{2-Oxo-Hexahydro-1H-Thieno[3,4-D]Imidazol-4-Yl}Pentanoate is a high-purity, activated ester derivative of biotin, specifically designed for bioconjugation and labeling applications. This compound is a critical reagent for researchers and manufacturers requiring reliable and efficient covalent attachment of biotin to biomolecules like proteins, peptides, and nucleic acids. It is essential for the development of diagnostic assays, targeted drug delivery systems, and advanced biochemical research tools in the pharmaceutical, biotechnology, and life sciences sectors.

Application

• Bioconjugation Reagent: Primary use for the covalent labeling of proteins, antibodies, and peptides with biotin for detection and purification.
• Diagnostic Assay Development: Key component in manufacturing ELISA kits, lateral flow assays, and other immunoassays that utilize streptavidin-biotin technology.
• Affinity Purification Systems: Used to create biotinylated ligands for immobilization on streptavidin-coated resins or surfaces.
• Drug Delivery & Targeting: Enables the biotinylation of drug carriers, nanoparticles, and targeting moieties in advanced therapeutic research.
• Cell Biology & Imaging: Facilitates the labeling of cell surface receptors or other cellular components for visualization and tracking studies.
• Nucleic Acid Labeling: Employed in the preparation of biotinylated probes for techniques like FISH (Fluorescence In Situ Hybridization) and microarray analysis.

Basic Information

Product Name	2,5-Dioxopyrrolidin-1-Yl 5-{2-Oxo-Hexahydro-1H-Thieno[3,4-D]Imidazol-4-Yl}Pentanoate
CAS No.	85718-04-3
Molecular Formula	C16H21N3O5S
Molecular Weight	367.42 g/mol
Synonyms	Biotin N-hydroxysuccinimide ester; Biotin NHS ester; D-Biotin N-succinimidyl ester; (+)-Biotin N-succinimidyl ester; Biotin succinimidyl ester; Biotin-OSu; NHS-Biotin; 5-[(3aS,4S,6aR)-2-Oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl]pentanoic acid N-succinimidyl ester
EINECS	Contact for details

Quality Control

Our 2,5-Dioxopyrrolidin-1-Yl 5-{2-Oxo-Hexahydro-1H-Thieno[3,4-D]Imidazol-4-Yl}Pentanoate is manufactured under strict quality management systems to ensure batch-to-batch consistency and high purity suitable for research and diagnostic applications. Each lot undergoes rigorous analytical testing, including HPLC for purity and identity confirmation. Certificates of Analysis (COA) detailing all specifications are provided with every shipment to support your quality assurance and regulatory compliance needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This compound is hygroscopic (moisture-sensitive) and light-sensitive. For long-term stability, store in a freezer and allow the sealed vial to equilibrate to room temperature before opening to prevent moisture condensation.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF; slightly soluble in acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.