Description

Succinyl-Alanyl-Alanyl-Phenylalanyl-4-Methoxy-2-Naphthylamide is a specialized synthetic peptide substrate designed for high-precision biochemical research and diagnostic applications. This compound is critical for studying and quantifying the activity of specific proteolytic enzymes, particularly elastase and related serine proteases. It is primarily utilized by pharmaceutical R&D laboratories, diagnostic kit manufacturers, and academic research institutions focused on enzymology, inflammation pathways, and protease inhibitor development.

Application

• Enzyme Activity Assays: Primary substrate for the fluorometric or colorimetric determination of human leukocyte elastase (HLE) and pancreatic elastase activity.
• Diagnostic Reagent Manufacturing: A key component in the formulation of clinical diagnostic kits for conditions involving elevated elastase levels, such as pancreatitis and certain inflammatory diseases.
• Pharmaceutical Research: Used in high-throughput screening (HTS) platforms to identify and evaluate novel elastase inhibitors for therapeutic development in pulmonary (e.g., COPD, cystic fibrosis) and inflammatory disorders.
• Biochemical Research: Essential tool for kinetic studies, enzyme characterization, and mechanistic investigations in academic and industrial enzymology laboratories.
• Quality Control in Biologics: Employed in QC labs to monitor protease activity during the production and purification of biopharmaceuticals.

Basic Information

Product Name	Succinyl-Alanyl-Alanyl-Phenylalanyl-4-Methoxy-2-Naphthylamide
CAS No.	85613-77-0
Molecular Formula	C₃₄H₄₁N₅O₈
Molecular Weight	647.73 g/mol
Synonyms	Suc-AAPF-4M-2NA; Suc-Ala-Ala-Pro-Phe-4-Methoxy-2-Naphthylamide; N-Succinyl-Ala-Ala-Pro-Phe-4-Methoxy-β-naphthylamide; Elastase Substrate IV; Leukocyte Elastase Substrate; Suc-AAPF-MNA; Protease Substrate S-2484 (analogous); N-Succinyl-L-alanyl-L-alanyl-L-prolyl-L-phenylalanine 4-methoxy-2-naphthylamide
EINECS	Contact for details

Quality Control

Our Succinyl-Alanyl-Alanyl-Phenylalanyl-4-Methoxy-2-Naphthylamide is manufactured under strict quality systems to ensure batch-to-batch consistency and reliability for sensitive research applications. Each lot undergoes comprehensive analytical testing including HPLC for purity, identity confirmation by mass spectrometry (MS) and NMR, and functional validation in enzymatic assays. A detailed Certificate of Analysis (COA) documenting purity, assay results, and physical characteristics is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. The product is hygroscopic (moisture-sensitive); allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis. For long-term storage, keep desiccated under inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Specific Rotation	Contact for details
Functional Activity	≥ 95% of reference standard in elastase assay
Solubility	Soluble in DMSO, DMF; slightly soluble in methanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.