Description

Polyethylene Glycol Bis(Succinimidyl Succinate) is a heterobifunctional crosslinking reagent featuring an amine-reactive N-hydroxysuccinimide (NHS) ester at each terminus, connected by a hydrophilic polyethylene glycol (PEG) spacer. This compound is critical for creating stable, water-soluble conjugates between biomolecules, such as proteins and peptides, while minimizing aggregation and preserving biological activity. It is an essential tool for researchers and manufacturers in bioconjugation, pharmaceutical development, and advanced material science, enabling the creation of next-generation therapeutics and diagnostic agents.

Application

• Protein-Protein and Protein-Peptide Crosslinking: For creating stable, defined conjugates for structural studies, immunoassays, and therapeutic antibody-drug complexes.
• PEGylation of Biomolecules: To modify therapeutic proteins, peptides, or small molecule drugs, enhancing their solubility, stability, and circulation half-life in vivo.
• Surface Functionalization: For immobilizing proteins or other ligands onto sensor chips, nanoparticles, or chromatography matrices for biosensing and diagnostic applications.
• Hydrogel and Biomaterial Synthesis: As a crosslinker in the development of PEG-based hydrogels for drug delivery systems and tissue engineering scaffolds.
• Antibody-Drug Conjugate (ADC) Linker Chemistry: Serving as a component in the linker-payload system to connect cytotoxic drugs to monoclonal antibodies.
• Diagnostic Reagent Preparation: For labeling antibodies, enzymes, or other proteins with fluorescent dyes, enzymes, or other reporter molecules.

Basic Information

Product Name	Polyethylene Glycol Bis(Succinimidyl Succinate)
CAS No.	85419-94-9
Molecular Formula	CnH2n+2On+8N2 (Variable based on PEG chain length 'n')
Molecular Weight	Variable (Contact for details on specific PEG chain length)
Synonyms	PEG-bis(NHS); NHS-PEG-NHS; Poly(ethylene glycol) bis(succinimidyl succinate); PEG-diol-bis(Succinimidyl Succinate); Succinimidyl PEG Succinimidyl; Bis-N-succinimidyl PEG ester; SS-PEG-SS; α,ω-Bis[N-succinimidyl(oxycarbonyl)ethyl]polyethylene glycol
EINECS	Contact for details

Quality Control

Our Polyethylene Glycol Bis(Succinimidyl Succinate) is manufactured under strict quality systems. Each batch undergoes rigorous analytical testing to ensure high purity and consistent performance for critical research and development applications. Certificates of Analysis (COA) detailing purity, functional group substitution, and residual solvent levels are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a freezer at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive). Allow the sealed vial to equilibrate to room temperature before opening to minimize condensation and hydrolysis of the active NHS ester groups.

Specification

Item	Specification
Appearance	White to off-white powder or solid
Identification (IR)	Conforms to structure
Assay (HPLC)	≥95% (NHS ester content)
PEG Average Molecular Weight	As specified (e.g., 2kDa, 3.4kDa, 5kDa)
Water Content (KF)	≤1.0%
Solubility	Freely soluble in DMF, DMSO; soluble in acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.