Description

2'(3')-o-(n-Methyl)Anthraniloylguanosine 5'-Triphosphate is a chemically modified nucleotide analog, specifically a fluorescently labeled GTP derivative. This compound is critical for advanced biochemical research and diagnostic assay development due to its unique fluorescent properties. It is primarily utilized by researchers in molecular biology, enzymology, and high-throughput screening for studying GTP-binding proteins, polymerases, and other nucleotide-dependent processes.

Application

• Biochemical Research: As a fluorescent substrate for studying the kinetics and mechanisms of GTPase and GTP-binding protein activities.
• Enzyme Assay Development: For creating sensitive, homogeneous assays to screen for inhibitors or activators of enzymes that utilize GTP, such as polymerases and signal transduction proteins.
• Fluorescence Spectroscopy & Microscopy: Used as a probe in FRET (Förster Resonance Energy Transfer) and other fluorescence-based techniques to monitor real-time conformational changes in proteins.
• Diagnostic Kit Manufacturing: A key component in the formulation of research-use-only (RUO) and in vitro diagnostic (IVD) kits for detecting specific enzymatic activities.
• High-Throughput Screening (HTS): Enables the development of robust, fluorescent readouts for drug discovery campaigns targeting nucleotide-binding sites.
• Nucleic Acid Labeling: Can be incorporated into oligonucleotides for creating fluorescent probes for hybridization and detection applications.

Basic Information

Product Name	2'(3')-o-(n-Methyl)Anthraniloylguanosine 5'-Triphosphate
CAS No.	85287-57-6
Molecular Formula	C23H27N8O16P3
Molecular Weight	788.43 g/mol
Synonyms	Mant-GTP; N-Methylanthraniloyl-GTP; 2'(3')-O-(N-Methylanthraniloyl)guanosine-5'-triphosphate; 2'/3'-O-(N-Methylanthraniloyl)-GTP; Fluorescent GTP; Mant-guanosine triphosphate; GTP, 2'(3')-O-[N-methylanthraniloyl]-; Guanosine-5'-triphosphate, 2'(3')-O-[N-methylanthraniloyl]-
EINECS	Contact for details

Quality Control

Every batch of 2'(3')-o-(n-Methyl)Anthraniloylguanosine 5'-Triphosphate is manufactured and tested under stringent quality protocols to ensure high purity, consistent performance, and batch-to-batch reproducibility essential for sensitive research applications. Our quality system includes verification of identity, purity (HPLC), and functional activity in standard assay formats. A comprehensive Certificate of Analysis (COA) detailing all specifications and test results is provided with each shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below. This product is hygroscopic (moisture-sensitive). For long-term storage, it is recommended to store desiccated at -80°C. Allow the vial to equilibrate to room temperature before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	Off-white to light yellow solid
Identification (HPLC)	Conforms
Purity (HPLC)	≥ 95%
Assay (Enzymatic)	≥ 90%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in aqueous buffer, DMSO

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.