Description

3'-O-(N-Methylanthraniloyl) ATP is a fluorescently labeled adenosine triphosphate (ATP) analog, widely used as a vital biochemical tool in enzymatic and binding studies. Its core value lies in its ability to serve as a high-sensitivity, non-radioactive substrate for monitoring ATPase and kinase activity in real-time. This compound is essential for researchers and developers in pharmaceutical discovery, molecular biology, and enzymology who require precise, fluorescent-based detection methods.

Application

• Real-time ATPase Activity Assays: A primary fluorescent substrate for monitoring the hydrolysis activity of various ATPase enzymes, including motor proteins like myosin and kinesin.
• Kinase Activity Screening: Used in high-throughput screening (HTS) platforms to identify and characterize kinase inhibitors for drug discovery.
• Biomolecular Interaction Analysis: Employed in fluorescence polarization (FP) and fluorescence resonance energy transfer (FRET) assays to study protein-ligand and protein-protein interactions.
• Enzymatic Mechanism Studies: Facilitates the detailed kinetic analysis of enzyme mechanisms that utilize ATP as a cofactor or substrate.
• Diagnostic Assay Development: Serves as a key component in developing sensitive diagnostic kits for diseases linked to aberrant ATPase or kinase function.
• Cellular Energy Metabolism Research: A tool for investigating ATP turnover and energy dynamics within cellular systems using fluorescence microscopy or plate readers.

Basic Information

Product Name	3'-O-(N-Methylanthraniloyl) ATP
CAS No.	85287-56-5
Molecular Formula	C24H28N6O14P3
Molecular Weight	717.43 g/mol
Synonyms	MANT-ATP; 3'-O-(N-Methyl-2-anthraniloyl)adenosine-5'-triphosphate; 3'-O-(N-Methylanthraniloyl)adenosine-5'-triphosphate; N-Methylanthraniloyl-ATP; 3'-MANT-ATP; Fluorescent ATP analog; 2'-(or-3')-O-(N-Methylanthraniloyl)adenosine 5'-triphosphate; Adenosine 5'-(tetrahydrogen triphosphate), 2'-(or-3')-O-[2-(methylamino)benzoyl]-, (triammonium salt)
EINECS	Contact for details

Quality Control

Our 3'-O-(N-Methylanthraniloyl) ATP is produced under stringent conditions to ensure high purity and batch-to-batch consistency, suitable for sensitive research applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and fluorescence properties. We adhere to rigorous internal quality standards to guarantee product integrity and performance in your assays.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive) and light-sensitive; allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	Off-white to yellow solid
Identification (HPLC)	Conforms
Purity (HPLC)	≥ 95%
Fluorescence Properties	Excitation ~356 nm, Emission ~448 nm (in aqueous buffer)
Water Content (KF)	≤ 5.0 %

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.