Description

3'-o-Anthraniloyl Atp is a fluorescently labeled adenosine triphosphate (ATP) analog, a critical biochemical tool for real-time enzymatic studies. This compound matters because its anthraniloyl fluorophore enables sensitive, non-radioactive detection of ATPase and kinase activity, providing superior signal-to-noise ratios. Researchers in biochemistry, molecular pharmacology, and drug discovery need it for high-throughput screening, mechanistic enzyme studies, and developing assays for nucleotide-binding proteins.

Application

• Enzyme Kinetics & Mechanism Studies: As a fluorescent substrate for continuous monitoring of ATP hydrolysis by various ATPases, kinases, and motor proteins.
• High-Throughput Screening (HTS): Development of fluorescence-based assays for drug discovery targeting nucleotide-dependent enzymes.
• Biomolecular Interaction Analysis: Probing binding events and conformational changes in proteins that interact with ATP, utilizing fluorescence polarization (FP) or fluorescence resonance energy transfer (FRET).
• Diagnostic Assay Development: Creating sensitive detection kits for clinical and research applications to measure enzymatic activity in biological samples.
• Cellular Energy Metabolism Research: Investigating ATP turnover and energy dynamics within live cells or cellular extracts using fluorescence microscopy or plate readers.

Basic Information

Product Name	3'-o-Anthraniloyl Atp
CAS No.	85287-49-6
Molecular Formula	C22H27N6O15P3
Molecular Weight	708.42 g/mol
Synonyms	3'-O-(2-Aminobenzoyl)adenosine 5'-triphosphate; 3'-O-Anthraniloyl-ATP; 3'-O-ABA-ATP; Anthraniloyl-ATP; 2'-Deoxy-3'-O-anthraniloyl-ATP (common misnomer, verify structure); Fluorescent ATP analog; AATP (context-dependent); ATP, 3'-O-[2-(aminobenzoyl)]-
EINECS	Contact for details

Quality Control

Our 3'-o-Anthraniloyl Atp is manufactured under strict quality protocols to ensure high purity and batch-to-batch consistency, suitable for sensitive research applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and performance in standard assays. We adhere to ISO 9001 quality management principles, and our production environment is designed to meet the exacting standards of life science research.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a desiccated environment. This compound is hygroscopic (moisture-sensitive) and light-sensitive (store away from light). For long-term stability, store under inert atmosphere. Allow the vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (HPLC)	Retention time corresponds to reference standard
Identification (MS)	Mass spectrum consistent with molecular structure
Purity (HPLC)	≥ 95%
Fluorescence Properties	Excitation ~320 nm, Emission ~410 nm (solvent dependent)
Water Content (KF)	≤ 3.0%
Biological Activity	Functions as substrate for reference ATPase (e.g., myosin)
Solubility	Soluble in aqueous buffer (pH ~7), DMSO

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.