Description

4-Methylumbelliferyl Phenylphosphonate is a specialized fluorogenic substrate and chemical intermediate of significant interest in biochemical research and diagnostics. This compound is valued for its role as a sensitive probe for detecting and quantifying the activity of specific enzymes, particularly phosphatases and esterases. It is primarily utilized by researchers and manufacturers in the life sciences, pharmaceutical development, and diagnostic reagent sectors for assay development and high-throughput screening applications.

Application

• Enzyme Activity Assays: A key fluorogenic substrate for the detection and kinetic study of phosphohydrolase and phosphatase enzymes.
• Diagnostic Reagent Manufacturing: Used in the formulation of diagnostic kits for clinical and research laboratories to measure specific enzymatic activities.
• High-Throughput Screening (HTS): Employed in pharmaceutical and biotechnology research for drug discovery campaigns targeting enzyme inhibition or activation.
• Biochemical Research: Serves as a critical tool for studying enzyme mechanisms, inhibition constants (Ki), and catalytic efficiency in vitro.
• Fluorophore-Labeled Probe Synthesis: Acts as a key intermediate in the synthesis of more complex fluorogenic or chromogenic substrates for advanced assays.

Basic Information

Product Name	4-Methylumbelliferyl Phenylphosphonate
CAS No.	85197-20-2
Molecular Formula	C16H13O6P
Molecular Weight	332.25 g/mol
Synonyms	4-Methylumbelliferyl Phenyl Phosphate; 7-Hydroxy-4-methylcoumarin Phenyl Phosphate; 4-MU-Phenylphosphate; Phenyl 4-Methylumbelliferyl Phosphate; 4-Methylumbelliferyl Phenylphosphonic Acid Ester; 4-MUPP; Fluorogenic Phenylphosphate Substrate
EINECS	Contact for details

Quality Control

Our 4-Methylumbelliferyl Phenylphosphonate is produced under strict quality management protocols to ensure high purity and batch-to-batch consistency, suitable for sensitive research and diagnostic applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles. We adhere to relevant quality standards for fine chemicals and can provide material tailored to specific research-grade requirements upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C or as indicated on the label or COA. This material is hygroscopic (moisture-sensitive) and light-sensitive (store away from light); ensure containers are sealed tightly after each use to minimize exposure to atmospheric moisture and light.

Specification

Item	Specification
Appearance	White to off-white powder or crystalline solid
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95% (Research Grade)
Water Content (KF)	≤ 2.0%
Fluorogenic Activity	Functional in standard phosphatase assays

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.