Description

5'-O-(4,4'-Dimethoxytrityl)-2'-deoxythymidine-3'-(methyl-N,N-diisopropyl)phosphoramidite is a critical phosphoramidite monomer used in the automated solid-phase synthesis of oligonucleotides. This high-purity building block is essential for the precise and efficient construction of DNA sequences, ensuring high coupling efficiency and yield. It is a fundamental raw material for research institutions, diagnostic kit manufacturers, and pharmaceutical companies engaged in the development of antisense oligonucleotides, primers, probes, and therapeutic nucleic acids.

Application

• Oligonucleotide Synthesis: As a thymidine (T) monomer for the automated, solid-phase synthesis of DNA strands.
• Therapeutic Nucleic Acid Development: Production of antisense oligonucleotides (ASOs), siRNA, and aptamers for pharmaceutical research and manufacturing.
• Diagnostic Probe Manufacturing: Synthesis of labeled and unlabeled DNA probes used in PCR, qPCR, microarrays, and in-situ hybridization kits.
• Primer Production: Fabrication of gene-specific primers for sequencing, cloning, and amplification techniques.
• Gene Synthesis: Building block for the de novo chemical synthesis of long DNA fragments and synthetic genes.
• Research & Development: Fundamental reagent in academic and industrial labs for molecular biology, genomics, and biotechnology studies.

Basic Information

Item	Detail
Product Name	5'-O-(4,4'-Dimethoxytrityl)-2'-deoxythymidine-3'-(methyl-N,N-diisopropyl)phosphoramidite
CAS No.	84416-85-3
Molecular Formula	C41H52N3O8P
Molecular Weight	745.85 g/mol
Synonyms	dT Phosphoramidite (DMT-off); 5'-O-DMT-Thymidine-3'-phosphoramidite; Thymidine Phosphoramidite; dT-CE Phosphoramidite; N,N-Diisopropylmethylphosphonamidic Chloride 2-Cyanoethyl Ester of 5'-O-(4,4'-Dimethoxytrityl)thymidine; T Phosphoramidite; 2'-Deoxythymidine Phosphoramidite
EINECS	Contact for details

Quality Control

Our phosphoramidite monomers are manufactured under strict quality systems to ensure consistency and reliability for sensitive oligonucleotide synthesis. Each batch is subjected to rigorous analytical testing, including HPLC, 31P NMR, and 1H NMR, to confirm identity, purity, and the absence of critical impurities. A comprehensive Certificate of Analysis (COA) detailing purity, moisture content, and performance data is provided with every shipment to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (argon or nitrogen) in a dry, moisture-free environment. This compound is hygroscopic (moisture-sensitive) and easily oxidized. Allow the sealed vial to equilibrate to room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC)	Conforms
Identification (31P NMR)	Conforms
Purity (HPLC)	≥ 98.0%
Moisture (Karl Fischer)	≤ 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.