Description

5'-O-(4,4'-Dimethoxytrityl)-N2-Isobutyryl-2'-Deoxyguanosine-3'-(Methyl-N,N-Diisopropyl)Phosphoramidite is a high-purity, protected nucleoside phosphoramidite monomer essential for automated solid-phase oligonucleotide synthesis. This compound is critical for ensuring high coupling efficiency and sequence fidelity in the production of DNA strands. It is primarily required by biotechnology, pharmaceutical, and diagnostic industries for applications in therapeutic oligonucleotide development, genetic research, and molecular diagnostics.

Application

• Oligonucleotide Synthesis: Key building block for the automated solid-phase synthesis of DNA sequences, including primers, probes, and antisense oligonucleotides.
• Therapeutic Development: Used in the research and manufacturing of siRNA, antisense drugs, aptamers, and other therapeutic oligonucleotides.
• Genetic Research & Diagnostics: Essential for producing custom DNA probes and primers used in PCR, sequencing, genotyping, and other molecular biology techniques.
• Synthetic Biology: Enables the construction of synthetic genes, gene fragments, and other engineered nucleic acid constructs.
• CRISPR/Cas9 Guide RNA Production: Used in the synthesis of custom guide RNA (gRNA) components for genome editing applications.
• DNA Microarray Fabrication: Serves as a precursor for in-situ synthesis of high-density DNA probes on microarray chips.

Basic Information

Product Name	5'-O-(4,4'-Dimethoxytrityl)-N2-Isobutyryl-2'-Deoxyguanosine-3'-(Methyl-N,N-Diisopropyl)Phosphoramidite
CAS No.	84416-84-2
Molecular Formula	C46H55N6O8P
Molecular Weight	851.0 g/mol
Synonyms	dG-Ibu Phosphoramidite; N2-Isobutyryl-5'-O-(4,4'-dimethoxytrityl)-2'-deoxyguanosine-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite (common alternative); Guanosine, 2'-deoxy-N2-(2-methyl-1-oxopropyl)-5'-O-[bis(4-methoxyphenyl)phenylmethyl]-, 3'-[2-cyanoethyl bis(1-methylethyl)phosphoramidite]; DMTr-dG(ib)-CE Phosphoramidite; 5'-O-DMT-N2-Ibu-2'-deoxyguanosine-3'-CE Phosphoramidite; dG(Ibu) Phosphoramidite; G(Ib) Phosphoramidite
EINECS	Contact for details

Quality Control

Our 5'-O-(4,4'-Dimethoxytrityl)-N2-Isobutyryl-2'-Deoxyguanosine-3'-(Methyl-N,N-Diisopropyl)Phosphoramidite is manufactured under strict quality systems to meet the exacting standards of oligonucleotide synthesis. Each batch undergoes rigorous analytical testing, including HPLC for purity and 31P NMR for identity and phosphitylation confirmation, to ensure optimal coupling performance. Certificates of Analysis (COA) with detailed chromatographic data are provided to guarantee traceability, consistency, and reliability for your critical research and GMP manufacturing processes.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (argon or nitrogen) in a dry, moisture-free environment. This material is hygroscopic (moisture-sensitive) and sensitive to strong bases and organic solvents. Allow the sealed vial to equilibrate to room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC)	Conforms
Identification (31P NMR)	Conforms
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.