Description

5'-Dimethoxytrityl-N4-Benzoyl-2'-Deoxycytidine-3'-(Methyl-N,n-Diisopropyl)Phosphoramidite is a high-purity, protected nucleoside phosphoramidite monomer essential for the automated solid-phase synthesis of oligonucleotides. Its primary value lies in enabling the precise and efficient incorporation of deoxycytidine into custom DNA sequences, which is critical for research, diagnostics, and therapeutic development. This building block is a fundamental requirement for biotechnology, pharmaceutical R&D, and molecular diagnostics companies engaged in the production of primers, probes, and synthetic genes.

Application

• Oligonucleotide Synthesis: As a key monomer for the automated, solid-phase synthesis of DNA strands in research and commercial settings.
• Therapeutic Oligonucleotide Production: Manufacturing of antisense oligonucleotides (ASOs), siRNA, aptamers, and other nucleic acid-based therapeutics.
• Diagnostic Probe Fabrication: Synthesis of labeled probes for PCR, qPCR, microarrays, fluorescence in situ hybridization (FISH), and other molecular diagnostic assays.
• Primer Synthesis for Sequencing & PCR: Production of high-fidelity primers used in next-generation sequencing (NGS), Sanger sequencing, and various polymerase chain reaction applications.
• Gene Synthesis and Cloning: Building block for the de novo synthesis of complete genes and gene fragments for synthetic biology and cloning workflows.
• Research & Development: Fundamental reagent in academic and industrial labs for studying nucleic acid chemistry, structure, and function.

Basic Information

Product Name	5'-Dimethoxytrityl-N4-Benzoyl-2'-Deoxycytidine-3'-(Methyl-N,n-Diisopropyl)Phosphoramidite
CAS No.	84416-83-1
Molecular Formula	C46H53N4O8P
Molecular Weight	828.91 g/mol
Synonyms	dC(Bz) Phosphoramidite; 5'-DMT-N4-Benzoyl-deoxycytidine-3'-phosphoramidite; N4-Benzoyl-5'-O-(4,4'-dimethoxytrityl)-2'-deoxycytidine-3'-[2-cyanoethyl-N,N-diisopropyl]phosphoramidite (common CE variant); DMTr-dC(Bz)-CE Phosphoramidite; Deoxycytidine Phosphoramidite, N4-Benzoyl protected; 2'-Deoxycytidine, 5'-O-[bis(4-methoxyphenyl)phenylmethyl]-N4-benzoyl-, 3'-[2-cyanoethyl N,N-diisopropylphosphoramidite]; DNA Synthesis Monomer dC(Bz)
EINECS	Contact for details

Quality Control

Our 5'-Dimethoxytrityl-N4-Benzoyl-2'-Deoxycytidine-3'-(Methyl-N,n-Diisopropyl)Phosphoramidite is manufactured under strict quality systems to ensure batch-to-batch consistency and high purity, suitable for demanding oligonucleotide synthesis. Each lot undergoes comprehensive analytical testing, including HPLC purity assay, 31P NMR and 1H NMR for structural confirmation, and moisture content analysis. Certificates of Analysis (COA) detailing all specifications are provided to guarantee compliance with your research or cGMP production requirements.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (argon or nitrogen) in a dry, moisture-free environment. This product is hygroscopic (moisture-sensitive) and easily oxidized; prolonged exposure to air, light, or ambient humidity will compromise stability and coupling efficiency. Allow the sealed vial to equilibrate to room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC)	Conforms to reference standard
Identification (31P NMR)	Conforms to reference standard
Purity (HPLC)	≥ 98.5%
Water Content (KF)	≤ 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.