Description

5'-O-(4,4'-Dimethoxytrityl)-N6-Benzoyl-2'-Deoxyadenosine-3'-(Methyl-N,N-Diisopropyl)Phosphoramidite is a high-purity, protected nucleoside phosphoramidite monomer essential for automated solid-phase oligonucleotide synthesis. Its primary value lies in enabling the precise, sequential addition of deoxyadenosine building blocks to create custom DNA sequences with high coupling efficiency and minimal side reactions. This critical reagent is indispensable for researchers and manufacturers in the pharmaceutical, biotechnology, and diagnostic industries who require reliable, high-quality inputs for synthesizing primers, probes, and therapeutic oligonucleotides. The compound is identified by CAS No. 84416-82-0.

Application

• Oligonucleotide Synthesis: As a key monomer for the automated solid-phase synthesis of DNA strands in research and commercial settings.
• Therapeutic Oligonucleotide Manufacturing: Production of antisense oligonucleotides (ASOs), siRNA, and aptamers for drug development and clinical applications.
• Diagnostic Probe Production: Synthesis of labeled and unlabeled DNA probes used in PCR, qPCR, microarrays, and next-generation sequencing (NGS).
• Primer Synthesis: Manufacturing of custom primers for genetic testing, molecular biology research, and forensic analysis.
• Gene Synthesis and Cloning: Building blocks for the de novo synthesis of genes and gene fragments.
• CRISPR Guide RNA Synthesis: Component in the synthesis of DNA templates for guide RNA production used in genome editing.

Basic Information

Product Name	5'-O-(4,4'-Dimethoxytrityl)-N6-Benzoyl-2'-Deoxyadenosine-3'-(Methyl-N,N-Diisopropyl)Phosphoramidite
CAS No.	84416-82-0
Molecular Formula	C47H55N6O7P
Molecular Weight	822.96 g/mol
Synonyms	dA(Bz) Phosphoramidite; N6-Benzoyl-5'-O-(4,4'-dimethoxytrityl)-2'-deoxyadenosine-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite; 5'-O-DMT-N6-benzoyl-2'-deoxyadenosine-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite; dA-CE Phosphoramidite (Bz protected); Deoxyadenosine Phosphoramidite (N6-Bz, 5'-DMT); Amidite dA(Bz); DNA Synthesis Monomer dA; Contact for additional synonyms.
EINECS	Contact for details

Quality Control

Every batch of our phosphoramidite monomers is produced under strict quality management systems to ensure the consistency and purity required for sensitive oligonucleotide synthesis. We provide comprehensive Certificates of Analysis (COA) detailing purity (typically ≥98% by HPLC), moisture content, and spectroscopic identity confirmation (NMR, MS). Our quality commitment aligns with the standards expected for GMP-grade starting materials used in therapeutic applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (argon or nitrogen) in a desiccated environment. This compound is hygroscopic (moisture-sensitive) and light-sensitive. Allow the sealed vial to equilibrate to room temperature before opening to prevent moisture condensation. For long-term stability, store in the original sealed packaging.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC)	Retention time corresponds to reference standard
Identification (NMR)	Spectrum consistent with structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 0.5%
Solubility	Clear, colorless solution in anhydrous acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.