Description

5-Hydroxyvitamin D3 25-Glucuronide is a key conjugated metabolite of vitamin D3, formed via glucuronidation at the 25-hydroxyl position. This compound is essential for research into the metabolic pathways, pharmacokinetics, and biological activity of vitamin D and its derivatives. It serves as a critical analytical standard and reference material for laboratories in the pharmaceutical, clinical diagnostics, and nutritional science sectors.

Application

• Biomedical Research Standard: Used as a certified reference material (CRM) for studying vitamin D metabolism, catabolism, and excretion pathways.
• Clinical Diagnostics Development: Serves as a primary calibrator and quality control standard in the development and validation of LC-MS/MS assays for accurate quantification of vitamin D metabolites in biological samples.
• Pharmacokinetic & ADME Studies: Employed in drug discovery and development to investigate the absorption, distribution, metabolism, and excretion (ADME) profiles of vitamin D-based therapeutics.
• Nutritional Science: A vital tool for research into vitamin D status, bioavailability, and the physiological role of conjugated metabolites.
• Biochemical Reagent: Used in enzymatic studies related to UDP-glucuronosyltransferase (UGT) activity and glucuronidation processes.

Basic Information

Product Name	5-Hydroxyvitamin D3 25-Glucuronide
CAS No.	84344-78-5
Molecular Formula	C₃₃H₅₂O₉
Molecular Weight	592.76 g/mol
Synonyms	25-Hydroxyvitamin D3 25-Glucuronide; 25(OH)D3-25-Gluc; 25-Hydroxycholecalciferol 25-Glucuronide; (3β,5Z,7E)-9,10-Secocholesta-5,7,10(19)-triene-3,25-diol 25-β-D-Glucopyranosiduronic Acid; Vitamin D3 Metabolite Glucuronide; 25-Glucuronide of 25-Hydroxyvitamin D3
EINECS	Contact for details

Quality Control

Our 5-Hydroxyvitamin D3 25-Glucuronide is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation via mass spectrometry, to ensure it meets the high standards required for research and diagnostic applications. A detailed Certificate of Analysis (COA) containing batch-specific data is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry, moisture-controlled environment. For long-term storage, consider storage under inert atmosphere or desiccant to maintain stability. Allow the product to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/MS)	Conforms to reference standard
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in methanol, DMSO; slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.