Description

4-Methylumbelliferyl-β-D-Lactoside is a specialized fluorogenic substrate used extensively in enzymatic assays. This compound is critical for researchers and quality control laboratories requiring high-sensitivity detection of β-galactosidase activity. It is primarily utilized in molecular biology, diagnostic kit manufacturing, and pharmaceutical research for applications such as gene expression studies and microbial detection. The product offers reliable performance due to its specific cleavage by the target enzyme, releasing the highly fluorescent 4-methylumbelliferone.

Application

• Enzymatic Assay Substrate: A key fluorogenic substrate for the detection and quantification of β-galactosidase (β-gal) enzyme activity in vitro.
• Molecular Biology Research: Used in reporter gene assays (e.g., lacZ gene expression) to study transcriptional regulation and promoter activity.
• Diagnostic Manufacturing: Incorporated into kits and reagents for clinical diagnostics, particularly for detecting enzymatic markers associated with certain genetic disorders or microbial contamination.
• Microbiology & Food Safety: Employed in assays to identify Escherichia coli and other coliform bacteria based on their β-galactosidase production.
• Pharmaceutical QC: Serves as a critical component in quality control testing for biopharmaceuticals where β-galactosidase is used as a processing enzyme or needs to be absent.
• High-Throughput Screening (HTS): Utilized in drug discovery platforms to screen for inhibitors or activators of β-galactosidase activity.

Basic Information

Product Name	4-Methylumbelliferyl-β-D-Lactoside
CAS No.	84325-23-5
Molecular Formula	C20H24O13
Molecular Weight	472.40 g/mol
Synonyms	4-MU-β-D-Lactoside; 4-Methylumbelliferyl β-D-galactopyranosyl-(1→4)-β-D-glucopyranoside; 4-Methylumbelliferyl lactoside; MULac; β-Galactosidase substrate 4-MU-β-D-Lactoside; 4-MU-Lactoside; 4-Methylumbelliferyl-β-D-galactopyranosyl-(1→4)-D-glucopyranoside
EINECS	Contact for details

Quality Control

Our 4-Methylumbelliferyl-β-D-Lactoside is manufactured under strict quality systems to ensure batch-to-batch consistency and high purity suitable for sensitive research and diagnostic applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and performance in standard enzymatic assays. We adhere to relevant industry guidelines for biochemical reagents.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent absorption of atmospheric moisture. For long-term storage, consider desiccants.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Fluorogenic Activity	Conforms to reference standard
Solubility	Soluble in DMSO, DMF; slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.