Description

4-Methylumbelliferyl-§-D-Cellopentoside is a specialized fluorogenic substrate used for the sensitive detection and quantification of cellulase enzyme activity. This compound is critical for research and industrial processes where precise measurement of cellulose degradation is required. It is primarily utilized by biotechnologists, enzymologists, and quality control laboratories in the biofuel, pulp & paper, textile, and animal feed industries to assay endo-1,4-β-glucanase activity.

Application

• Cellulase Enzyme Assays: A standard fluorogenic substrate for the sensitive, continuous measurement of endo-1,4-β-glucanase (cellulase) activity in research and industrial settings.
• Biofuel Production Research: Used to screen and optimize microbial strains or enzyme cocktails for the efficient saccharification of lignocellulosic biomass.
• Pulp & Paper Industry: Employed in quality control and process development to monitor cellulase activity in enzymatic pulp treatment and deinking processes.
• Textile Processing: Applied in the development and monitoring of enzymatic bio-polishing and stone-washing treatments for cellulose-based fabrics like cotton.
• Animal Feed Additive Analysis: Used to assay the activity of cellulase enzymes added to animal feed to improve digestibility of fibrous materials.
• Diagnostic Kit Development: Serves as a key component in the formulation of diagnostic kits and reagents for enzymatic analysis in clinical and food testing laboratories.
• High-Throughput Screening (HTS): Ideal for automated, microplate-based HTS of compound libraries for cellulase inhibitors or activators in drug discovery and agrochemical research.

Basic Information

Product Name	4-Methylumbelliferyl-§-D-Cellopentoside
CAS No.	84325-20-2
Molecular Formula	C34H42O21
Molecular Weight	786.69 g/mol
Synonyms	4-MU-β-D-cellopentoside; 4-Methylumbelliferyl β-D-cellopentoside; 4-Methylumbelliferyl β-D-cellopentoside; MU-Cellopentoside; 4-MU-Cellopentoside; Fluorogenic cellulase substrate; 4-Methylumbelliferyl-β-D-cellopentaose
EINECS	Contact for details

Quality Control

Our 4-Methylumbelliferyl-§-D-Cellopentoside is manufactured under strict quality control protocols to ensure high purity and consistent performance in sensitive enzymatic assays. Each batch is characterized by HPLC, NMR, and mass spectrometry to confirm identity and purity. Certificates of Analysis (COA) detailing purity, assay results, and impurity profiles are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry environment. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 2.0%
Fluorogenic Activity	Conforms to reference standard
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.