Description

4-Methylumbelliferyl-§-D-Cellotetroside is a specialized fluorogenic substrate designed for the sensitive detection and quantification of cellulase enzyme activity. This compound matters because it provides a highly specific and reliable method for analyzing the hydrolysis of cellotetraose, a key intermediate in cellulose breakdown. Researchers and quality control laboratories in the biotechnology, biofuel, and enzyme production industries need it to optimize processes, screen for novel enzymes, and ensure product consistency.

Application

• Cellulase Enzyme Assays: Primary substrate for the fluorometric measurement of endo-1,4-β-glucanase (cellulase) activity.
• Biomass Conversion Research: Used in studies to evaluate the efficiency of enzymatic cocktails for lignocellulosic biomass degradation in biofuel production.
• Enzyme Kinetics & Characterization: Essential tool for determining kinetic parameters (Km, Vmax) and inhibition studies of cellulolytic enzymes.
• High-Throughput Screening (HTS): Employed in industrial and academic settings to screen microbial libraries or mutant enzymes for enhanced cellulolytic activity.
• Quality Control in Enzyme Manufacturing: Standardized reagent for batch-to-batch consistency testing of commercial cellulase preparations.
• Diagnostic & Clinical Research: Potential use in research assays related to microbial infections where cellulase is a biomarker.

Basic Information

Product Name	4-Methylumbelliferyl-§-D-Cellotetroside
CAS No.	84325-19-9
Molecular Formula	C34H44O23
Molecular Weight	820.70 g/mol
Synonyms	4-MU-β-D-cellotetraoside; 4-Methylumbelliferyl β-D-cellotetraoside; 4-Methylumbelliferyl β-cellotetraoside; MU-Cellotetraose; 4-MU-Cellotetraoside; Fluorogenic cellotetraose substrate; 7-Hydroxy-4-methylcoumarin-β-D-cellotetraoside
EINECS	Contact for details

Quality Control

Our 4-Methylumbelliferyl-§-D-Cellotetroside is produced under stringent quality protocols to ensure high purity and batch-to-batch reproducibility, critical for sensitive enzymatic assays. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and performance in standard assay conditions. We adhere to ISO 9001 quality management principles, and our manufacturing process is designed to meet the exacting requirements of research and industrial applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a desiccated environment. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/IR)	Conforms to reference standard
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 2.0%
Fluorogenic Activity	Conforms to performance standard
Solubility	Soluble in water, DMSO, DMF

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.