Description

4-Methylumbelliferyl Pyrophosphate Diester Disodium Salt is a high-purity, synthetic biochemical substrate of significant importance in enzymatic research and diagnostic assay development. Its core value lies in its role as a fluorogenic substrate for enzymes like glycosyltransferases and phosphatases, where enzymatic cleavage releases the highly fluorescent 4-methylumbelliferone (4-MU). This compound is essential for research institutions, diagnostic kit manufacturers, and pharmaceutical companies engaged in high-throughput screening, enzyme kinetics studies, and the development of sensitive clinical diagnostics.

Application

• Enzymatic Assay Substrate: Primary fluorogenic substrate for measuring the activity of various enzymes, including glycosyltransferases, sulfotransferases, and specific phosphatases.
• Diagnostic Kit Manufacturing: Critical raw material in the production of diagnostic kits for lysosomal storage disorders (e.g., Gaucher disease, Fabry disease) and other enzymatic deficiency assays.
• Biochemical Research: Used in fundamental research to study enzyme mechanisms, inhibition, and kinetics in vitro.
• High-Throughput Screening (HTS): Employed in drug discovery platforms to identify potential therapeutic compounds that modulate specific enzyme targets.
• Quality Control in Biopharma: Used to test the enzymatic activity of recombinant enzyme batches during biopharmaceutical production.

Basic Information

Product Name	4-Methylumbelliferyl Pyrophosphate Diester Disodium Salt
CAS No.	84282-11-1
Molecular Formula	C11H11Na2O11P2
Molecular Weight	426.10 g/mol
Synonyms	4-MU-PP Disodium Salt; 4-Methylumbelliferyl Diphosphate Disodium Salt; MUPP Disodium Salt; 4-Methylumbelliferyl Pyrophosphate Disodium; 7-Hydroxy-4-methyl-2-oxo-2H-1-benzopyran-8-yl Diphosphate Disodium Salt; 4-Methylumbelliferyl Diphosphoric Acid Ester Disodium Salt; 4-MU-Diphosphate Disodium Salt
EINECS	Contact for details

Quality Control

Our 4-Methylumbelliferyl Pyrophosphate Diester Disodium Salt is manufactured under strict quality systems to ensure batch-to-batch consistency and high performance in sensitive applications. Each lot undergoes rigorous analytical testing, including HPLC for purity and assay, spectral identification (IR, NMR), and tests for residual solvents and moisture. A comprehensive Certificate of Analysis (COA) detailing all specifications is provided with every shipment to support your quality assurance and regulatory requirements.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to reference spectrum
Identification (HPLC)	Retention time matches reference standard
Assay (HPLC)	≥ 95.0%
Purity (HPLC)	≥ 98.0% (Area %)
Water Content (KF)	≤ 5.0%
Solubility	Clear, colorless solution in water
Functional Activity	Conforms to reference in standard enzyme assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.