Description

n-Dansyl-D-Ala-Gly-4-Nitro-Phe-Gly CAS NO 83960-27-4 is a specialized, fluorescently labeled peptide derivative designed for advanced biochemical research and development. This compound is valued for its role as a high-precision tool in enzyme kinetics and proteomics studies, where its dansyl fluorophore and nitro-phenylalanine moiety enable sensitive detection and analysis. It is primarily utilized by research institutions, pharmaceutical R&D departments, and diagnostic kit manufacturers focused on protease activity assays and fluorescence-based detection systems.

Application

• Protease & Enzyme Substrate: Acts as a specific, sensitive fluorogenic or chromogenic substrate for studying the kinetics and inhibition of various proteolytic enzymes.
• Biochemical Research: Used in fundamental research to investigate enzyme mechanisms, protein-protein interactions, and signal transduction pathways.
• Pharmaceutical Screening: Employed in high-throughput screening (HTS) assays to identify and characterize potential therapeutic enzyme inhibitors.
• Diagnostic Development: Serves as a key component in developing fluorescence-based diagnostic assays for clinical and research laboratories.
• Peptide Chemistry Studies: Utilized as a model compound in studies of peptide stability, fluorescence quenching, and intramolecular interactions.

Basic Information

Product Name	n-Dansyl-D-Ala-Gly-4-Nitro-Phe-Gly
CAS No.	83960-27-4
Molecular Formula	C30H34N6O9S
Molecular Weight	654.70 g/mol
Synonyms	Dansyl-D-alanylglycyl-4-nitrophenylalanylglycine; Dns-D-Ala-Gly-pNO2-Phe-Gly; N-[[4-[[(2,5-Dimethylphenyl)sulfonyl]amino]butanoyl]-D-alanylglycyl-4-nitrophenylalanylglycine; 5-Dimethylaminonaphthalene-1-sulfonyl-D-alanyl-glycyl-4-nitro-L-phenylalanyl-glycine; Fluorescent Peptide Substrate 83960-27-4; Dns-DAGpNFG; N-Dansyl-D-alanyl-glycyl-4-nitro-L-phenylalanyl-glycine
EINECS	Contact for details

Quality Control

Our n-Dansyl-D-Ala-Gly-4-Nitro-Phe-Gly is manufactured under strict quality systems to ensure batch-to-batch consistency and high purity for critical research applications. Each lot is characterized by advanced analytical techniques including HPLC, MS, and NMR to confirm identity and purity. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to warm to room temperature in a desiccator before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	Yellow to light brown powder
Identification (HPLC)	Conforms
Identification (MS)	Conforms
Purity (HPLC)	≥ 95.0%
Assay	≥ 97.0% (on dried basis)
Water Content (KF)	≤ 3.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.