Description

Benzyloxycarbonylglycyl-Phenylalanyl-Citrulline 4-Nitroanilide is a specialized synthetic peptide substrate designed for enzymatic research and diagnostic applications. This compound is critical for the study and quantification of proteolytic enzyme activity, particularly serine proteases. It is an essential reagent for pharmaceutical R&D, clinical diagnostics, and biochemical laboratories focused on enzyme kinetics and inhibitor screening.

Application

• Protease Activity Assays: Primary substrate for the colorimetric or fluorometric detection and quantification of specific serine protease activity.
• Drug Discovery & Development: Used in high-throughput screening (HTS) platforms to identify and characterize novel protease inhibitors for therapeutic targets.
• Diagnostic Kit Manufacturing: A key component in the formulation of in-vitro diagnostic (IVD) kits for clinical analysis of enzyme levels.
• Biochemical Research: Essential tool for studying enzyme kinetics, mechanism of action, and substrate specificity in academic and industrial research settings.
• Quality Control in Biologics: Employed in purity and potency testing of enzyme-based biopharmaceutical products.

Basic Information

Product Name	Benzyloxycarbonylglycyl-Phenylalanyl-Citrulline 4-Nitroanilide
CAS No.	83575-50-2
Molecular Formula	C30H32N8O8
Molecular Weight	632.63 g/mol
Synonyms	Z-Gly-Phe-Cit-pNA; Z-Gly-Phe-Cit-4-NA; Cbz-Gly-Phe-Cit-pNA; Benzyloxycarbonyl-Gly-Phe-Citrulline-p-Nitroanilide; Z-Gly-Phe-Cit-p-Nitroanilide; Substrate for Serine Proteases; Protease Substrate 83575-50-2
EINECS	Contact for details

Quality Control

Our Benzyloxycarbonylglycyl-Phenylalanyl-Citrulline 4-Nitroanilide is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity, to ensure it meets the high standards required for research and diagnostic applications. A comprehensive Certificate of Analysis (COA) detailing identity, purity, and assay results is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive); allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms
Identification (Mass Spectrometry)	Conforms
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.