Description

H-Ala-Arg-Amc Hcl is a specialized fluorogenic peptide substrate designed for high-sensitivity protease activity assays. This compound is critical for biochemical research and pharmaceutical development, enabling precise measurement of enzyme kinetics and inhibitor screening. It is primarily utilized by researchers and quality control laboratories in the life sciences, biotechnology, and drug discovery sectors.

Application

• Protease Activity Assays: As a fluorogenic substrate for measuring the activity of specific proteases, including trypsin-like enzymes.
• Enzyme Kinetic Studies: Used in high-throughput screening (HTS) to determine kinetic parameters (Km, Vmax) of target enzymes.
• Drug Discovery & Development: A key tool for screening and characterizing potential protease inhibitors in pharmaceutical R&D.
• Biochemical Research: Employed in academic and industrial labs to study protease function, signaling pathways, and proteolytic cascades.
• Diagnostic Reagent Development: Serves as a component in developing sensitive diagnostic kits for detecting protease-related conditions.
• Quality Control Testing: Used in biomanufacturing to monitor and validate the activity of enzyme-based products.

Basic Information

Product Name	H-Ala-Arg-Amc Hcl
CAS No.	83363-71-7
Molecular Formula	C20H30N8O4 • HCl
Molecular Weight	506.98 g/mol
Synonyms	Ala-Arg-AMC hydrochloride; H-Ala-Arg-7-amido-4-methylcoumarin hydrochloride; L-Alanyl-L-arginyl-7-amido-4-methylcoumarin hydrochloride; Boc-Ala-Arg-AMC HCl (common precursor name); Fluorogenic substrate for trypsin; AMC substrate Ala-Arg; Protease substrate 83363-71-7; AAF-AMC related substrate
EINECS	Contact for details

Quality Control

Our H-Ala-Arg-Amc Hcl is manufactured under strict quality management systems to ensure batch-to-batch consistency and reliability for sensitive research applications. Each lot undergoes comprehensive analytical testing, including HPLC purity analysis, identity confirmation by mass spectrometry, and verification of fluorogenic activity. Certificates of Analysis (COA) detailing all specifications are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake. For long-term stability, store under inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Fluorogenic Activity	Functional in standard assay
Solubility	Soluble in DMSO, DMF; slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.