Description

Pglu-Pro-Val-Paranitroanilide is a specialized synthetic peptide substrate designed for biochemical research and diagnostic applications. This compound is critical for the study and quantification of specific protease enzyme activity, offering high sensitivity and selectivity. It is primarily utilized by research institutions, pharmaceutical R&D departments, and diagnostic kit manufacturers focused on enzymology and biomarker discovery.

Application

• Protease Enzyme Assays: Used as a chromogenic substrate for the kinetic study and activity measurement of specific proteolytic enzymes, such as dipeptidyl peptidase IV (DPP-IV) and related enzymes.
• Biochemical Research: Serves as a key reagent in fundamental research to understand enzyme kinetics, inhibition mechanisms, and substrate specificity.
• Pharmaceutical Screening: Employed in high-throughput screening (HTS) platforms to identify and evaluate potential therapeutic enzyme inhibitors for drug discovery programs.
• Diagnostic Development: Integral component in the development of in vitro diagnostic (IVD) tests and kits for clinical analysis of enzyme levels related to specific physiological or pathological conditions.
• Quality Control in Biologics: Applied in the quality control testing of biopharmaceuticals to monitor the presence and activity of process-related enzymes.

Basic Information

Product Name	Pglu-Pro-Val-Paranitroanilide
CAS No.	83329-36-6
Molecular Formula	C₂₀H₂₆N₆O₆
Molecular Weight	446.46 g/mol
Synonyms	L-Pyroglutamyl-L-prolyl-L-valine 4-nitroanilide; Pyr-Gly-Pro-Val-pNA; DPP-IV Substrate; H-Gly-Pro-Val-pNA; Dipeptidyl Peptidase IV Substrate; GPV-pNA; 4-Nitroanilide of L-Pyroglutamyl-L-prolyl-L-valine; Pyr-GPV-pNA
EINECS	Contact for details

Quality Control

Our Pglu-Pro-Val-Paranitroanilide is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity verification, to ensure it meets the high standards required for sensitive research and diagnostic applications. Certificates of Analysis (COA) with detailed chromatographic data are provided to guarantee traceability and batch-to-batch consistency.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. Due to its hygroscopic (moisture-sensitive) nature, the container must be kept sealed in a desiccated environment to maintain stability and prevent degradation.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.5%
Single Impurity (HPLC)	≤ 0.5%
Total Impurities (HPLC)	≤ 2.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.