Description

Methylsulfonylleucyl-Glycyl-Arginine-4-Nitroanilide is a synthetic tripeptide derivative containing a chromogenic 4-nitroanilide leaving group. This compound is primarily valued as a highly specific and sensitive chromogenic substrate for the detection and quantification of serine protease activity. It is essential for researchers and quality control laboratories in the pharmaceutical and biotechnology sectors who require precise enzymatic assays for drug development, diagnostic applications, and biochemical research.

Application

• Enzymatic Assay Substrate: A specific chromogenic substrate for the kinetic study and inhibition profiling of serine proteases, including thrombin, trypsin, and related enzymes.
• Pharmaceutical Research: Used in high-throughput screening (HTS) campaigns to identify and characterize novel protease inhibitors for therapeutic development.
• Diagnostic Reagent Manufacturing: A key component in the formulation of diagnostic kits for measuring coagulation factors and other protease-related clinical markers.
• Biochemical Research: Employed in academic and industrial laboratories to investigate protease signaling pathways, enzyme mechanisms, and substrate specificity.
• Quality Control (QC) Testing: Utilized in the QC of biopharmaceuticals, such as recombinant proteins, to ensure the absence of residual protease activity.

Basic Information

Product Name	Methylsulfonylleucyl-Glycyl-Arginine-4-Nitroanilide
CAS No.	83160-48-9
Molecular Formula	C23H37N9O8S
Molecular Weight	599.66 g/mol
Synonyms	Methylsulfonyl-Leu-Gly-Arg-pNA; Ms-Leu-Gly-Arg-pNA; Tosyl-Leu-Gly-Arg-pNA (common variant); Thrombin Substrate I; Chromogenic Substrate for Thrombin; H-D-Leu-Gly-Arg-pNA Tosylate Salt (related salt form); Boc-Leu-Gly-Arg-pNA (related protected form); 4-Nitroanilide of Methylsulfonylleucylglycylarginine
EINECS	Contact for details

Quality Control

Our Methylsulfonylleucyl-Glycyl-Arginine-4-Nitroanilide is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for research and diagnostic applications. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and related substances is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This product is hygroscopic (moisture-sensitive) and should be handled in a dry environment to prevent degradation. For long-term storage, desiccants are recommended.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Assay	95.0% - 105.0%
Water Content (KF)	≤ 2.0%
Residue on Ignition	≤ 0.5%
Solubility	Soluble in DMSO, DMF; slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.