Description

Leukotriene B4, Methyl Ester is a chemically modified derivative of the potent pro-inflammatory lipid mediator Leukotriene B4 (LTB4). This methyl ester form is a crucial research-grade biochemical used extensively in analytical and pharmacological studies. It serves as a key reference standard and synthetic intermediate for scientists in pharmaceutical R&D and life sciences. This compound is essential for researchers investigating inflammatory pathways, developing enzyme inhibitors, and conducting mass spectrometry-based lipidomics.

Application

• Biochemical Research Standard: Primary use as a certified reference material (CRM) for the quantification and identification of LTB4 and its metabolites in biological samples via LC-MS/MS.
• Pharmaceutical Development: Critical intermediate in the synthesis of novel anti-inflammatory drug candidates targeting the LTB4 pathway, such as BLT receptor antagonists.
• Enzyme Activity Studies: Used as a substrate or inhibitor in assays to study the activity of enzymes like leukotriene A4 hydrolase (LTA4H).
• Cell Signaling & Inflammation Research: Employed in vitro and ex vivo to study neutrophil chemotaxis, immune cell activation, and other inflammatory responses mediated by LTB4 receptors.
• Metabolite Profiling: Essential for creating calibration curves and validating analytical methods in lipid mediator metabolomics and eicosanoid profiling.
• Quality Control: Serves as an internal standard for the pharmaceutical quality control of related therapeutic compounds.

Basic Information

Product Name	Leukotriene B4, Methyl Ester
CAS No.	83058-42-8
Molecular Formula	C21H34O4
Molecular Weight	350.49 g/mol
Synonyms	LTB4 Methyl Ester; Methyl (5S,6Z,8E,10E,12R,14Z)-5,12-Dihydroxyicosa-6,8,10,14-tetraenoate; 5(S),12(R)-Dihydroxy-6,8,10,14-eicosatetraenoic Acid Methyl Ester; Methyl 5,12-DiHETE; (5S,6Z,8E,10E,12R,14Z)-5,12-Dihydroxyicosa-6,8,10,14-tetraenoic Acid Methyl Ester
EINECS	Contact for details

Quality Control

Our Leukotriene B4, Methyl Ester is produced under strict quality management systems to ensure batch-to-batch consistency and high purity for research applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity confirmation, and impurity profiles. We adhere to relevant standards for research chemicals and analytical reference materials. Certificates of Analysis (COA) are available upon request.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C or below under an inert atmosphere (e.g., argon or nitrogen) to prevent oxidation. Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture condensation. Store away from strong acids and oxidizing agents.

Specification

Item	Specification
Appearance	White to off-white solid or oil
Identification (HPLC)	Conforms to reference standard
Identification (MS)	Conforms to reference standard
Purity (HPLC)	≥ 95% (Area %)
Assay	≥ 90% (by weight)
Solubility	Soluble in ethanol, methanol, DMSO; slightly soluble in aqueous buffers

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.