Description

Testosterone (3,4-13C2) is a stable isotope-labeled analog of the primary male sex hormone, testosterone, specifically labeled with carbon-13 at the 3 and 4 positions. This precise labeling makes it an indispensable internal standard and tracer in quantitative mass spectrometry-based analyses, ensuring unparalleled accuracy and reliability in research data. It is critically needed by pharmaceutical R&D laboratories, clinical research organizations, and forensic toxicology labs for applications in drug metabolism studies, doping control, endocrine research, and the development of advanced diagnostic assays.

Application

• Internal Standard for LC-MS/MS: Primary use as a quantitative reference standard in liquid chromatography-tandem mass spectrometry for accurate measurement of endogenous testosterone levels in biological matrices (serum, plasma, urine).
• Drug Metabolism and Pharmacokinetics (DMPK): Tracer for studying the absorption, distribution, metabolism, and excretion (ADME) profiles of testosterone and related anabolic-androgenic steroids.
• Clinical Diagnostics & Endocrine Research: Essential for developing and validating high-precision clinical assays to diagnose hypogonadism, monitor hormone replacement therapy, and investigate endocrine disorders.
• Sports Doping Control: Critical reference material for anti-doping laboratories worldwide to confirm the presence of exogenous testosterone and its metabolites, supporting fair competition.
• Biochemical Research: Tool for investigating androgen receptor binding, steroidogenic pathways, and the biochemical mechanisms of androgen action.
• Pharmaceutical Quality Control: Used in the analysis and quality assurance of testosterone-based pharmaceutical formulations.

Basic Information

Product Name	Testosterone (3,4-13C2)
CAS No.	82938-02-1
Molecular Formula	C19H2813C2O2
Molecular Weight	~290.44 g/mol
Synonyms	Testosterone-3,4-13C2; [3,4-13C2]-Testosterone; 17β-Hydroxyandrost-4-en-3-one-3,4-13C2; (8R,9S,10R,13S,14S,17S)-13-methyl-17-hydroxy-2,6,7,8,9,10,11,12,14,15,16,17-dodecahydro-1H-cyclopenta[a]phenanthren-3-one-3,4-13C2; Androst-4-en-17β-ol-3-one-3,4-13C2; Isotopically Labeled Testosterone; 13C2-Testosterone
EINECS	Contact for details

Quality Control

Every batch of Testosterone (3,4-13C2) is manufactured and analyzed under strict quality systems. We guarantee high chemical and isotopic purity, verified by advanced spectroscopic and chromatographic techniques including NMR, HPLC, and MS. A comprehensive Certificate of Analysis (COA) is provided with each shipment, detailing specifications for identity, purity (HPLC), isotopic enrichment, and residual solvents. Our quality commitment ensures the material meets the exacting standards required for regulatory submission and sensitive analytical applications.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C. This compound is hygroscopic (moisture-sensitive); the container must be kept tightly sealed in a cool, dry place to maintain stability and purity. For long-term storage, consider storage under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to reference spectrum
Identification (MS)	Conforms to expected mass spectrum
Purity (HPLC)	≥ 98.0%
Isotopic Enrichment	≥ 99 atom % 13C (at 3,4 positions)
Chemical Purity (by NMR)	Conforms
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.