Description

n-Hydroxysulfosuccinimide (CAS NO 82436-78-0) is a highly reactive, water-soluble heterobifunctional crosslinking agent and activating reagent. Its core value lies in its ability to facilitate the efficient conjugation of biomolecules, such as proteins and peptides, under mild aqueous conditions, which is critical for maintaining biological activity. This compound is essential for researchers and manufacturers in the fields of bioconjugation, diagnostics, and therapeutic development who require reliable and high-purity reagents for creating stable, functional conjugates.

Application

• Bioconjugation Reagent: Used as a key intermediate for activating carboxyl groups on proteins, antibodies, and other biomolecules for subsequent coupling to amines, enabling the creation of antibody-drug conjugates (ADCs), enzyme conjugates, and labeled proteins.
• Diagnostic Kit Manufacturing: Employed in the development of immunoassays (ELISA, lateral flow tests) to conjugate detection enzymes (e.g., HRP) or fluorescent dyes to antibodies and antigens.
• Biomaterial and Hydrogel Synthesis: Serves as a crosslinker for modifying polymers and hydrogels with bioactive peptides or proteins to create functional scaffolds for tissue engineering and drug delivery systems.
• Surface Immobilization Chemistry: Facilitates the covalent attachment of biomolecules to activated surfaces on biosensors, microarrays, and chromatography media.
• Peptide Synthesis and Modification: Used in solid-phase and solution-phase peptide synthesis for side-chain modification and the introduction of specific functional handles.
• Affinity Chromatography Ligand Coupling: Activates carboxylated matrices or ligands for the stable immobilization of proteins, enabling the purification of specific targets.

Basic Information

Product Name	n-Hydroxysulfosuccinimide
CAS No.	82436-78-0
Molecular Formula	C4H5NO7S
Molecular Weight	211.15 g/mol
Synonyms	Sulfo-NHS; N-Hydroxysulfosuccinimide sodium salt; Sulfosuccinimidyl ester; 1-(3-Sulfopropyl)-3-hydroxy-2,5-pyrrolidinedione; Sulfo-N-hydroxysuccinimide; NHS-Sulfonate; Water-soluble NHS ester; Sulfo-NHS ester
EINECS	Contact for details

Quality Control

Our n-Hydroxysulfosuccinimide is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity, NMR for structural confirmation, and titration for active group content, to ensure it meets the high standards required for sensitive life science applications. Certificates of Analysis (COA) with detailed specifications are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C (refrigerated) or as specified on the label. Due to its hygroscopic (moisture-sensitive) nature, the container must be kept sealed under anhydrous conditions and allowed to equilibrate to room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Solubility (in Water)	Clear, colorless solution
Heavy Metals	≤ 10 ppm
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.