Description

Suberate Bis(Sulfosuccinimidyl) Sodium Salt is a water-soluble, homobifunctional N-hydroxysulfosuccinimide (sulfo-NHS) ester crosslinker. This compound is specifically engineered for the efficient and gentle conjugation of biomolecules, particularly proteins and peptides, under physiological conditions. It is an essential reagent for researchers and manufacturers in the life sciences, bioconjugation, and diagnostic kit development sectors who require reliable, amine-reactive crosslinking agents.

Application

• Protein-Protein Conjugation: For creating stable antibody-enzyme complexes, fusion proteins, and other protein assemblies for immunoassays and therapeutic applications.
• Peptide Carrier Conjugation: Used to conjugate haptens or small peptide antigens to larger carrier proteins (e.g., BSA, KLH) to enhance immunogenicity.
• Biomolecule Immobilization: Facilitates the covalent attachment of proteins, antibodies, or other amine-containing ligands to amine-reactive surfaces on biosensors, microarrays, and chromatographic resins.
• Diagnostic Reagent Development: A key component in the synthesis of labeled probes and detection reagents for ELISA, lateral flow assays, and other clinical diagnostics.
• Polymer Functionalization: Enables the introduction of amine-reactive groups onto polymers or nanoparticles for subsequent bioconjugation in drug delivery and material science.
• Crosslinking for Structural Studies: Employed in studying protein interactions, complex formation, and spatial relationships through chemical crosslinking coupled with mass spectrometry (XL-MS).

Basic Information

Product Name	Suberate Bis(Sulfosuccinimidyl) Sodium Salt
CAS No.	82436-77-9
Molecular Formula	C20H24N2Na2O14S2
Molecular Weight	634.51 g/mol
Synonyms	BS3 (sodium salt); Bis(sulfosuccinimidyl) suberate sodium salt; Disodium 3,3'-((1,8-dioxooctane-1,8-diyl)bis(oxy))bis(2,5-dioxo-1,2,5-oxathiazolidine-4-sulfonate); Suberic acid bis(N-hydroxysulfosuccinimide) ester sodium salt; Sulfo-BS3; BS3, sulfo-; DTSSP (Analog); Homobifunctional sulfo-NHS ester crosslinker
EINECS	Contact for details

Quality Control

Our Suberate Bis(Sulfosuccinimidyl) Sodium Salt is manufactured under strict quality systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, NMR and MS for structural confirmation, and functional activity validation in standard conjugation assays. We provide a detailed Certificate of Analysis (COA) with every shipment, ensuring traceability and compliance with research-grade specifications. Custom synthesis and specific purity grades (e.g., >95%, >98%) are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake. For long-term stability, store under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 2.0%
Solubility	Freely soluble in water, DMSO
Functional Activity	Conforms (by amine conjugation test)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.