Description

Cystamine-Sepharose CAS NO 82346-95-0 is a specialized affinity chromatography medium, consisting of cystamine (2,2'-dithiobis(ethylamine)) covalently immobilized onto a cross-linked agarose (Sepharose) matrix. This product is engineered for the specific and reversible capture of thiol-containing biomolecules, enabling critical purification and isolation workflows. It is an essential tool for researchers and process developers in biopharmaceutical manufacturing, proteomics, and enzyme research, facilitating the study and production of proteins, peptides, and other thiolated compounds.

Application

• Purification of Thiol-Containing Proteins and Enzymes: Selective capture and elution via reversible disulfide bond formation for high-purity isolation.
• Antibody Fragment (Fab, scFv) Purification: Exploits free cysteine residues in antibody fragments for specific affinity chromatography.
• Immobilization of Cysteine-Tagged Recombinant Proteins: Serves as a solid support for immobilizing enzymes or proteins for activity studies or as reusable biocatalysts.
• Redox State Analysis and Thiol-Disulfide Exchange Studies: A critical tool for investigating protein folding, stability, and redox regulation mechanisms.
• Sample Preparation for Proteomics: Isolation and enrichment of cysteine-containing peptides from complex mixtures for mass spectrometry analysis.
• Removal of Reducing Agents: Can be used to scavenge or quantify low molecular weight thiols like dithiothreitol (DTT) or β-mercaptoethanol from solutions.
• Process Development in Biologics Manufacturing: Scalable purification step for therapeutic proteins that rely on disulfide bonds for correct structure.

Basic Information

Product Name	Cystamine-Sepharose
CAS No.	82346-95-0
Molecular Formula	N/A (Functionalized polymer matrix)
Molecular Weight	N/A (Matrix-based product)
Synonyms	2,2'-Dithiobis(ethylamine)-Sepharose
Cystamine immobilized on Sepharose
Sepharose-cystamine
Dithiobis(ethylamine)-agarose
Activated Thiol-Sepharose (precursor/form)
Disulfide-linked amine agarose
6-Aminohexyl-2-pyridyldithio-agarose (related activation product)
EINECS	Contact for details

Quality Control

Our Cystamine-Sepharose is produced under a quality-managed system to ensure batch-to-batch consistency for research and process applications. Each lot is characterized for key performance parameters, including ligand density, binding capacity, and matrix stability. Certificates of Analysis (COA) detailing these specifications are provided with every shipment. While not a drug substance, its manufacture considers principles relevant to GMP-grade ancillary materials used in bioprocessing.

Storage

Preserve in a tightly closed container, protected from light. Store as a 20% ethanol slurry or in recommended storage buffer at 2-8°C. Do not freeze. Under these conditions, the product is stable for the period indicated on the label. The matrix should be protected from drying out and from microbial contamination.

Specification

Item	Specification
Matrix	Cross-linked 4% or 6% Agarose (Sepharose 4B/6B)
Ligand	Cystamine (2,2'-Dithiobis(ethylamine))
Ligand Density	5 - 25 μmol/mL gel (Typical)
Binding Capacity	10 - 40 mg thiolated protein/mL gel
Particle Size	45 - 165 μm
Maximum Flow Pressure	0.1 MPa (1 bar)
pH Stability (long term)	3 - 12 (working)
Storage Form	20% Ethanol slurry or specified buffer

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.