Description

Methotrexate Polyglutamate is a polyglutamated metabolite of the antifolate drug methotrexate, representing a key active form with enhanced intracellular retention and potency. This compound is critical for advanced pharmacological research, particularly in understanding drug resistance and optimizing therapeutic regimens in oncology and autoimmune disease treatment. It is an essential reference standard and biochemical tool for pharmaceutical R&D laboratories, clinical research organizations, and academic institutions focused on cancer biology and targeted therapy development.

Application

• Pharmaceutical Reference Standard: Used as a high-purity calibrant in quantitative analytical methods (HPLC, LC-MS) for drug metabolism and pharmacokinetic (DMPK) studies.
• Biochemical Research: Serves as a critical reagent in investigating the mechanism of action, intracellular polyglutamation, and retention of methotrexate in cancer cells.
• Oncology Research: Employed in studies of antifolate resistance, folate pathway inhibition, and the development of novel chemotherapeutic strategies.
• Metabolite Analysis: Essential for the identification and quantification of methotrexate polyglutamates in biological matrices (plasma, tissue, red blood cells) in clinical and preclinical settings.
• Enzyme Substrate/Inhibitor Studies: Used in assays for enzymes like folylpolyglutamate synthetase (FPGS) and γ-glutamyl hydrolase (GGH).
• Therapeutic Drug Monitoring (TDM) Development: Aids in developing and validating assays to monitor methotrexate polyglutamate levels, which correlate with clinical efficacy and toxicity.

Basic Information

Product Name	Methotrexate Polyglutamate
CAS No.	82334-40-5
Molecular Formula	C24H32N8O9 (for a representative polyglutamate form; chain length may vary)
Molecular Weight	~576.56 g/mol (for diglutamate form)
Synonyms	MTX Polyglutamate; Methotrexate Diglutamate; Methotrexate Poly-γ-glutamate; L-Glutamic acid, N-[4-[[(2,4-diamino-6-pteridinyl)methyl]methylamino]benzoyl]-, (2S)-2-[[(1S)-1-[[4-[[(2,4-diamino-6-pteridinyl)methyl]methylamino]benzoyl]amino]-4-carboxy-1-oxobutyl]amino]pentanedioic acid; γ-Glutamyl-Methotrexate; Polyglutamyl Methotrexate; MTX-Glun
EINECS	Contact for details

Quality Control

Our Methotrexate Polyglutamate is produced and handled under strict quality management protocols. Each batch undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation (NMR, MS), to ensure it meets the exacting standards required for research and development. A detailed Certificate of Analysis (COA) is provided with each shipment, documenting purity, assay results, and chromatographic data.

Storage

Preserve in a tightly closed container, protected from light. Store at controlled room temperature (15-25°C) or as indicated on the label or COA. For long-term storage, consider storing desiccated at -20°C. This product is light-sensitive (store away from light).

Specification

Item	Specification
Appearance	Yellow to orange powder
Identification (HPLC)	Conforms to reference standard
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 5.0%
Residual Solvents (GC)	Complies with ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.