Description

4-(2-Hydroxyethyl)Piperazine-1-Ethanesulfonic Acid Potassium Salt is a high-purity, water-soluble potassium salt derivative of the widely used HEPES biological buffer. This compound matters for its superior buffering capacity in the physiological pH range (7.2-7.6), offering enhanced stability and minimal interference with biochemical systems. It is essential for researchers and manufacturers in biotechnology, pharmaceutical development, and cell culture media formulation who require precise and consistent pH control in sensitive applications.

Application

• Cell Culture & Tissue Culture Media: As a primary buffering agent to maintain stable pH in mammalian and bacterial cell cultures.
• Molecular Biology & Biochemistry: For pH stabilization in enzyme assays, protein purification, electrophoresis, and PCR reactions.
• Pharmaceutical Formulation: Used in the development and manufacturing of diagnostic reagents, vaccines, and other biopharmaceuticals.
• Diagnostic Kits & Reagents: As a key component in buffers for clinical diagnostic assays and test kits.
• Biochemical Research: Providing a non-volatile, zwitterionic buffer for a wide range of in vitro studies and experiments.
• IVD (In-Vitro Diagnostic) Manufacturing: Ensuring consistent performance and shelf-life of diagnostic solutions.

Basic Information

Product Name	4-(2-Hydroxyethyl)Piperazine-1-Ethanesulfonic Acid Potassium Salt
CAS No.	82207-62-3
Molecular Formula	C8H17KN2O4S
Molecular Weight	276.40 g/mol
Synonyms	HEPES Potassium Salt; Potassium HEPES; 2-[4-(2-Hydroxyethyl)piperazin-1-yl]ethanesulfonic Acid Potassium Salt; N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid) Potassium Salt; HEPES K+ Salt; EPPS Potassium Salt (common misnomer); PIPES derivative potassium salt; Biological Buffer Potassium Salt
EINECS	Contact for details

Quality Control

Our 4-(2-Hydroxyethyl)Piperazine-1-Ethanesulfonic Acid Potassium Salt is manufactured under strict quality systems. Each batch undergoes rigorous analytical testing to ensure it meets high-purity standards suitable for research and biopharmaceutical applications. Specifications typically align with industry benchmarks for biological buffers. A comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry, and well-ventilated area at room temperature (15-25°C). This product is hygroscopic (moisture-sensitive); ensure the container is sealed after each use to prevent absorption of atmospheric moisture.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms
Assay (HPLC)	≥ 99.0%
pH (1% Solution)	7.0 - 7.5
Water Content (KF)	≤ 1.0%
Heavy Metals (as Pb)	≤ 10 ppm
Endotoxin (LAL)	< 0.1 EU/mg (upon request)
Filterability (0.2 µm)	Clear, colorless solution

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.