Description

Restriction Endodeoxyribonuclease Hincii is a highly specific type II restriction enzyme that recognizes and cleaves the palindromic DNA sequence GTPyPuAC. This product is essential for molecular biology research, enabling precise DNA manipulation, mapping, and cloning. It is a critical reagent for biotechnology companies, academic research laboratories, and diagnostic kit manufacturers requiring reliable and high-activity enzymatic tools for genetic analysis.

Application

• Molecular Cloning: For the precise digestion of DNA fragments prior to ligation into vectors.
• Restriction Fragment Length Polymorphism (RFLP) Analysis: Used in genetic fingerprinting and mutation detection studies.
• DNA Mapping: Essential for constructing physical maps of genomes and plasmids.
• Recombinant DNA Technology: A fundamental tool for constructing recombinant DNA molecules in genetic engineering workflows.
• Diagnostic Assay Development: Incorporated into kits for the detection of specific genetic sequences or mutations.
• Academic & Industrial Research: Supports basic and applied research in genetics, genomics, and synthetic biology.

Basic Information

Product Name	Restriction Endodeoxyribonuclease Hincii
CAS No.	81811-55-4
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	HinciI; HinciI Restriction Enzyme; Endodeoxyribonuclease HinciI; Deoxyribonuclease HinciI; Restriction Enzyme HinciI; GTPyPuAC-specific Type II Restriction Enzyme; EC 3.1.21.4
EINECS	Contact for details

Quality Control

Our Restriction Endodeoxyribonuclease Hincii is produced under stringent quality management systems. Each lot undergoes rigorous activity, purity, and specificity testing to ensure it is free from contaminating nucleases and non-specific endonucleolytic activities. A comprehensive Certificate of Analysis (COA) detailing activity units, purity, and storage buffer composition is provided with every shipment to guarantee batch-to-batch consistency and performance for your critical applications.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C ± 5°C in a non-frost-free freezer. Avoid repeated freeze-thaw cycles. The enzyme is supplied in a stabilized storage buffer containing 50% glycerol.

Specification

Item	Specification
Appearance	Clear, colorless solution
Activity	≥ 10,000 units/mL
Purity	No detectable non-specific endonuclease or exonuclease activity
Specificity	Cleaves GTPyPuAC sites as specified
Storage Buffer	10 mM Tris-HCl, 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol (v/v), pH 7.4 at 25°C
Unit Definition	One unit is the amount of enzyme required to completely digest 1 µg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 µL.

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.