Description

Restriction Endonuclease Acc II is a type II restriction enzyme that recognizes and cleaves specific DNA sequences, serving as a critical tool in molecular biology research. Its high specificity and reliable activity are essential for applications such as DNA mapping, cloning, and genetic engineering. This enzyme is primarily utilized by biotechnology firms, academic research laboratories, and diagnostic kit manufacturers requiring precise DNA manipulation.

Application

• Molecular Cloning: For the precise cutting and insertion of DNA fragments into vectors.
• DNA Mapping: Used in restriction fragment length polymorphism (RFLP) analysis and genome mapping studies.
• Genetic Engineering: A key reagent for constructing recombinant DNA molecules.
• Diagnostic Assay Development: Employed in the creation of tests for genetic disorders and pathogen detection.
• Biotechnology Research: Essential for fundamental research in genomics, proteomics, and synthetic biology.
• Quality Control in Pharmaceutical Manufacturing: Used to verify the genetic identity of plasmid DNA and other biologics.

Basic Information

Product Name	Restriction Endonuclease Acc II
CAS No.	81811-50-9
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	AccII Restriction Enzyme; Endodeoxyribonuclease AccII; AccII (Acinetobacter calcoaceticus); Restriction Enzyme AccII; Type II Site-Specific Deoxyribonuclease AccII; ACC II; AccII Endonuclease; Acinetobacter calcoaceticus II
EINECS	Contact for details

Quality Control

Our Restriction Endonuclease Acc II is produced and tested under stringent conditions to ensure high purity, specific activity, and absence of contaminating nucleases. Each batch is validated for its cleavage specificity and reaction efficiency. A comprehensive Certificate of Analysis (COA), detailing activity units, purity assessments, and storage buffer composition, is provided to guarantee reproducible and reliable performance in your critical applications.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C ± 5°C in a non-frost-free freezer. Avoid repeated freeze-thaw cycles. The enzyme is supplied in a stabilized storage buffer; please do not dilute prior to storage.

Specification

Item	Specification
Appearance	Clear, colorless solution
Identification (Activity)	Cleaves DNA at recognition sequence 5'...CGCG...3'
Specific Activity	≥ 10,000 units/mg protein
Purity (SDS-PAGE)	≥ 95%
Contaminating Nuclease Activity	Not detected
Nickase Activity	Not detected
Storage Buffer	Tris-HCl, NaCl, EDTA, Glycerol, DTT

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.