Description

n-α-p-Tos-Gly-Pro-Arg-p-Nitroanilide is a specialized chromogenic peptide substrate designed for the selective detection and quantification of thrombin and other serine proteases. This compound matters for its high sensitivity and specificity in biochemical assays, enabling critical research and quality control in life sciences. It is primarily needed by pharmaceutical R&D laboratories, diagnostic kit manufacturers, and academic research institutions focused on hemostasis, thrombosis, and protease inhibitor screening.

Application

• Thrombin Activity Assays: Primary substrate for the colorimetric or spectrophotometric determination of thrombin activity in plasma samples and purified systems.
• Serine Protease Research: Used in kinetic studies and inhibitor screening for enzymes like trypsin, plasmin, and factor Xa.
• Diagnostic Development: A key component in the formulation of clinical diagnostic kits for coagulation disorders and liver function tests.
• Pharmaceutical QC: Employed in the quality control of heparin, direct thrombin inhibitors, and other anticoagulant drugs.
• Biochemical Research: Fundamental tool for studying enzyme kinetics, catalytic mechanisms, and substrate specificity of proteases.

Basic Information

Product Name	n-α-p-Tos-Gly-Pro-Arg-p-Nitroanilide
CAS No.	81790-80-9
Molecular Formula	C30H40N8O8S
Molecular Weight	672.76 g/mol
Synonyms	Tos-Gly-Pro-Arg-pNA; Chromozym TH; H-D-Phe-Pip-Arg-p-nitroanilide dihydrochloride (related); Thrombin Substrate I; p-Tosyl-Gly-Pro-Arg-p-Nitroanilide; Bz-Phe-Val-Arg-pNA (related); S-2238 (related); Benzoyl-Phe-Val-Arg-p-nitroanilide (related)
EINECS	Contact for details

Quality Control

Our n-α-p-Tos-Gly-Pro-Arg-p-Nitroanilide is manufactured under strict quality systems to ensure batch-to-batch consistency and high performance in sensitive assays. Each lot is supported by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and functional activity. We adhere to relevant industry standards for research-grade biochemicals, ensuring reliability for your critical applications.

Storage

Preserve in a tightly closed container, protected from light. Store at controlled room temperature (15-25°C) in a dry environment. Due to its hygroscopic (moisture-sensitive) nature, the container should be kept sealed and allowed to equilibrate to room temperature before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/MS)	Conforms
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Functional Activity	≥ 95% of reference standard
Solubility	Clear, colorless solution in DMSO or water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.