Description

Restriction Endonuclease Sau 3A I is a type II restriction enzyme isolated from *Staphylococcus aureus* strain 3A. This enzyme is essential for molecular biology research, specifically for DNA manipulation and recombinant DNA technology, due to its ability to recognize and cleave specific DNA sequences. It is a critical reagent for laboratories and companies engaged in genetic engineering, synthetic biology, and biopharmaceutical development, where precise DNA cutting is required for cloning, mapping, and analysis.

Application

• Molecular Cloning: Essential for creating recombinant DNA constructs by digesting DNA at specific recognition sites.
• Genomic DNA Analysis: Used in restriction mapping and Southern blotting to analyze genetic structure and polymorphisms.
• DNA Library Construction: Facilitates the preparation of genomic or cDNA libraries for sequencing and screening projects.
• Synthetic Biology: A key tool for assembling genetic circuits and synthetic pathways in engineered organisms.
• Biopharmaceutical R&D: Employed in the development of gene therapies, vaccines, and therapeutic protein production systems.
• Diagnostic Assay Development: Used to prepare DNA probes and targets for diagnostic kits and genetic testing.

Basic Information

Product Name	Restriction Endonuclease Sau 3A I
CAS No.	81725-92-0
Molecular Formula	Protein
Molecular Weight	Contact for details
Synonyms	Sau3AI; Endodeoxyribonuclease Sau3AI; Sau 3A; Restriction enzyme Sau3A I; Type II restriction enzyme Sau3AI; EC 3.1.21.4; Staphylococcus aureus 3A restriction endonuclease
EINECS	Contact for details

Quality Control

Our products undergo rigorous quality testing to ensure compliance with industry standards for molecular biology reagents. Each batch is tested for specific activity, purity, and the absence of contaminating nucleases and phosphatases. Certificates of Analysis (COA) detailing lot-specific performance data, including unit definition and optimal reaction conditions, are available upon request.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C in a non-frost-free freezer. Avoid repeated freeze-thaw cycles. The enzyme should be kept in the supplied storage buffer upon receipt.

Specification

Item	Specification
Appearance	Clear colorless solution
Identification (Activity)	Cleaves GATC sequences to produce expected DNA fragment pattern
Specific Activity	≥ 10,000 units/mg protein
Purity (SDS-PAGE)	≥ 95%
Contaminating Nuclease Activity	Not detected
Contaminating Phosphatase Activity	Not detected
Storage Buffer	10 mM Tris-HCl, 100 mM KCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, pH 7.4 at 25°C

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.