Description

β-Cyanoethyl-Adenosine 5'-(1-Thiodiphosphate) is a chemically modified nucleotide analog where a sulfur atom replaces one of the non-bridging oxygen atoms in the diphosphate moiety. This modification, along with the β-cyanoethyl protecting group, makes it a valuable non-hydrolyzable ATP analog and a crucial reagent in enzymology and molecular biology research. It is primarily utilized by researchers and manufacturers in the life sciences sector for studying ATP-dependent enzymes, kinase mechanisms, and signal transduction pathways. The compound's stability against phosphatases allows for precise investigation of phosphorylation events and enzyme kinetics.

Application

• Enzyme Kinetics Research: Used as a non-hydrolyzable or slow-turnover substrate analog to study the mechanisms of ATPases, kinases, and other ATP-binding enzymes.
• Structural Biology: Employed in X-ray crystallography and NMR studies to capture and determine the structures of enzyme-substrate complexes.
• Signal Transduction Studies: Applied in biochemical assays to investigate phosphorylation cascades and cellular signaling pathways without the complication of hydrolysis.
• Diagnostic Kit Development: Serves as a key component in the development of high-sensitivity assays for enzyme activity and drug screening platforms.
• Pharmaceutical Research: Utilized in target validation and inhibitor screening for drug discovery programs focused on kinase-related diseases.
• Biochemical Tool Synthesis: Acts as a precursor or intermediate for synthesizing more complex nucleotide probes and affinity labels.

Basic Information

Product Name	β-Cyanoethyl-Adenosine 5'-(1-Thiodiphosphate)
CAS No.	81655-27-8
Molecular Formula	C₁₄H₂₁N₆O₁₁P₂S
Molecular Weight	558.36 g/mol
Synonyms	Adenosine 5'-(1-thiodiphosphate) β-cyanoethyl ester; 5'-Adenylyl (β-cyanoethyl)phosphorothioate; ATP-γ-S β-cyanoethyl ester; β-CE-ATPgammaS; Adenosine 5'-[γ-thio]triphosphate, β-cyanoethyl ester; Non-hydrolyzable ATP-γ-S analog; ARC 660; β-Cyanoethyl-ATP-γ-S
EINECS	Contact for details

Quality Control

Our β-Cyanoethyl-Adenosine 5'-(1-Thiodiphosphate) is produced under stringent quality management protocols to ensure batch-to-batch consistency and high purity for critical research applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity assessment and NMR for structural confirmation. Certificates of Analysis (COA) detailing all specifications and test results are available upon request to support your quality assurance and regulatory documentation.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder or solid
Identification (HPLC)	Conforms
Identification (NMR)	Conforms
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in water, DMSO

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.