Description

Uv Endonuclease CAS NO 81611-73-6 is a specialized enzyme critical for DNA repair research and molecular biology applications. This product is essential for scientists studying nucleotide excision repair pathways and DNA damage response mechanisms. It is primarily used by research institutions, pharmaceutical R&D departments, and biotechnology companies engaged in genetic analysis and therapeutic development.

Application

• DNA Repair Mechanism Studies: Essential for in vitro assays investigating the nucleotide excision repair (NER) pathway, specifically for cleaving DNA at sites of UV-induced photolesions like cyclobutane pyrimidine dimers (CPDs).
• Molecular Biology Research: Used as a key reagent in enzymatic assays to map and quantify UV-induced DNA damage, aiding in the study of mutagenesis and carcinogenesis.
• Pharmaceutical R&D: Employed in drug discovery platforms targeting DNA repair enzymes, helping to screen and evaluate potential chemotherapeutic agents or radio-sensitizers.
• Genetic Toxicology Testing: Utilized in standardized tests to assess the genotoxic potential of chemicals, environmental agents, and new compounds by measuring their capacity to induce DNA damage repaired by this pathway.
• Biomarker Development: Supports research into developing clinical biomarkers for cancer susceptibility and prognosis based on individual DNA repair capacity.
• Enzymology and Protein Biochemistry: Serves as a model enzyme for studying enzyme kinetics, protein-DNA interactions, and the structure-function relationships of endonucleases.

Basic Information

Product Name	Uv Endonuclease
CAS No.	81611-73-6
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	UV-Specific Endonuclease; UV Endodeoxyribonuclease; Deoxyribonuclease (Pyrimidine Dimer); CPD-Specific Endonuclease; UV Damage Endonuclease; T4 Endonuclease V (homolog); Photolyase-independent DNA repair enzyme; EC 3.1.25.1 (formerly)
EINECS	Contact for details

Quality Control

Our Uv Endonuclease is produced under stringent quality management systems to ensure batch-to-batch consistency and optimal enzymatic activity. Each lot is tested for specific activity, purity (via SDS-PAGE), and the absence of contaminating nucleases and proteases. A comprehensive Certificate of Analysis (COA) detailing assay results, storage buffer composition, and unit definition is provided with every shipment to support your research reproducibility and compliance needs.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store frozen at -20°C or below in a non-frost-free freezer. Avoid repeated freeze-thaw cycles. Aliquot the product upon receipt for single-use applications to maintain maximum activity.

Specification

Item	Specification
Appearance	Clear, colorless to slightly yellow solution
Identification (Activity)	Cleaves supercoiled DNA containing UV-induced pyrimidine dimers
Specific Activity	≥ X,XXX units/mg protein (Contact for specific grade)
Purity (SDS-PAGE)	≥ 95%
Contaminating Activities	Free of detectable RNase, DNase (non-specific), and protease activities
Protein Concentration	Defined per batch (see COA)
Storage Buffer	Tris-HCl, Glycerol, DTT, EDTA (exact composition on COA)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.