Description

2-Hydroxyestrone-1,4,16,16-D4 is a high-purity, deuterium-labeled internal standard critical for advanced analytical chemistry. This compound is essential for ensuring the accuracy and precision of quantitative mass spectrometry analyses, particularly in complex biological matrices. It is primarily utilized by research institutions, pharmaceutical developers, and clinical diagnostic laboratories engaged in steroid hormone metabolism studies, pharmacokinetic research, and biomarker discovery.

Application

• Quantitative Mass Spectrometry (LC-MS/MS, GC-MS): Serves as a stable isotope-labeled internal standard for the precise quantification of endogenous 2-hydroxyestrone and related metabolites.
• Clinical Research & Diagnostics: Enables accurate measurement of estrogen metabolism profiles in studies related to oncology, endocrinology, and women's health.
• Pharmacokinetic & Drug Metabolism Studies: Used in ADME (Absorption, Distribution, Metabolism, Excretion) research for drugs targeting estrogen pathways.
• Biomarker Discovery & Validation: Facilitates the development of robust assays for potential diagnostic biomarkers in various disease states.
• Forensic & Anti-Doping Analysis: Supports method development for the detection of endogenous steroid hormones and their metabolites in forensic and sports testing.
• Reference Standard for Method Development: Provides a certified reference material for calibrating analytical instruments and validating new laboratory methods.

Basic Information

Item	Details
Product Name	2-Hydroxyestrone-1,4,16,16-D4
CAS No.	81586-97-2
Molecular Formula	C18H18D4O3
Molecular Weight	290.39 g/mol
Synonyms	1,3,5(10)-Estratrien-2,3-diol-17-one-1,4,16,16-D4; 2-OHE1-D4; 2-Hydroxyestrone-d4; [1,4,16,16-D4]-2-Hydroxyestrone; 2-Hydroxy-1,3,5(10)-estratrien-17-one-1,4,16,16-D4; Deuterated 2-Hydroxyestrone; Estrogen Metabolite-d4
EINECS	Contact for details

Quality Control

Every batch of 2-Hydroxyestrone-1,4,16,16-D4 is manufactured under strict quality management systems and undergoes comprehensive analytical characterization. Purity and isotopic enrichment are verified using state-of-the-art techniques including HPLC, NMR, and mass spectrometry. A detailed Certificate of Analysis (COA) is provided with each shipment, confirming identity, chemical purity, isotopic purity (D-enrichment), and absence of critical impurities.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Identification (MS)	Conforms to reference standard
Purity (HPLC)	≥ 98.0%
Isotopic Purity (D-enrichment)	≥ 98.0 atom % D
Chemical Purity (NMR)	Conforms
Water Content (KF)	≤ 1.0%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.