Description

23,25,26-Trihydroxyvitamin D3 is a biologically active metabolite of vitamin D3, representing a high-purity reference standard and key intermediate in pharmaceutical research. This compound is critical for studying the complex metabolic pathways and physiological roles of vitamin D beyond calcium homeostasis. It is primarily utilized by research institutions and pharmaceutical developers focused on endocrinology, bone metabolism, and novel therapeutic applications.

Application

• Pharmaceutical Reference Standard: Used as a high-purity calibrant in analytical methods (HPLC, LC-MS) for quantifying vitamin D metabolites in biological samples.
• Biochemical Research: Serves as a critical tool for investigating non-classical actions of vitamin D, including its effects on cell differentiation, proliferation, and immune modulation.
• Metabolic Pathway Studies: Essential for elucidating the catabolic pathways of 1,25-dihydroxyvitamin D3, the active hormonal form of vitamin D.
• Drug Discovery & Development: Acts as a key intermediate or precursor in the synthesis of novel vitamin D analogs for potential therapeutic use.
• Diagnostic Kit Manufacturing: Employed in the development and calibration of immunoassays and diagnostic tests for vitamin D status assessment.

Basic Information

Product Name	23,25,26-Trihydroxyvitamin D3
CAS No.	81515-15-3
Molecular Formula	C₂₇H₄₄O₅
Molecular Weight	448.64 g/mol
Synonyms	1α,25-Dihydroxy-23,26-lactone-27-norvitamin D3; 23,25,26-Trihydroxycholecalciferol; (1R,3S)-5-[2-[(1R,3aS,7aR)-1-[(2R)-6-hydroxy-6-methylheptan-2-yl]-7a-methyl-2,3,3a,5,6,7-hexahydro-1H-inden-4-ylidene]ethylidene]-4-methylidenecyclohexane-1,3-diol
EINECS	Contact for details

Quality Control

Our 23,25,26-Trihydroxyvitamin D3 is manufactured under strict quality management systems. Each batch is subjected to comprehensive analytical testing, including HPLC for purity and identity confirmation by spectroscopic methods (NMR, MS). We provide Certificates of Analysis (COA) detailing all specifications, ensuring traceability and compliance with research-grade standards. Stability studies are conducted to guarantee performance throughout the shelf life.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere. The product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the sealed vial to equilibrate to room temperature before opening to prevent condensation and moisture ingress.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.