Description

8-N3-Atp is a photoactivatable, azide-modified derivative of adenosine triphosphate (ATP) designed for cross-linking and labeling studies. This compound is a critical tool for researchers investigating ATP-binding proteins, enabling the covalent capture of protein-ligand interactions upon UV irradiation. It is essential for scientists in biochemistry, molecular biology, and proteomics who require precise mechanisms to study enzyme kinetics, protein function, and interaction networks.

Application

• Photoaffinity Labeling: Acts as a photoreactive probe to covalently tag and identify ATP-binding sites in proteins and enzymes.
• Proteomics Research: Used in activity-based protein profiling (ABPP) to discover and characterize novel ATP-interacting proteins.
• Enzyme Mechanism Studies: Facilitates the investigation of kinase, ATPase, and synthase mechanisms by trapping transient enzyme-substrate complexes.
• Bioconjugation & Probe Synthesis: Serves as a key reagent for synthesizing more complex chemical probes via click chemistry, leveraging the azide functional group.
• Drug Discovery: Employed in target identification and validation for therapeutic programs focused on ATP-competitive inhibitors.
• Structural Biology: Aids in the study of protein structures and dynamics by providing a means to stabilize and isolate ligand-bound complexes.

Basic Information

Product Name	8-N3-Atp
CAS No.	81380-25-8
Molecular Formula	C10H16N8O13P3
Molecular Weight	573.15 g/mol
Synonyms	8-Azidoadenosine 5'-triphosphate; 8-Azido-ATP; 8-N3ATP; Adenosine 5'-triphosphate, 8-azido-; 8-Azidoadenosine triphosphate; γ-Azido-ATP (positional isomer); Photoactivatable ATP; ATP azide derivative
EINECS	Contact for details

Quality Control

Every batch of 8-N3-Atp is manufactured and tested to meet stringent research-grade standards. Our quality assurance protocol includes comprehensive analytical characterization to ensure high purity, structural integrity, and functional activity. Certificates of Analysis (COA) detailing purity (HPLC), identity (NMR, MS), and performance are provided with each shipment to support your critical research applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a desiccated environment. This product is hygroscopic (moisture-sensitive) and light-sensitive. For long-term stability, store under inert atmosphere and avoid repeated freeze-thaw cycles.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 2.0%
Functional Activity	Tested in standard kinase assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.