Description

Xho I is a high-purity restriction endonuclease enzyme critical for precise DNA manipulation in molecular biology research and biotechnological applications. This enzyme recognizes the specific palindromic sequence 5'-C↓TCGAG-3' and cleaves to produce cohesive ends, enabling efficient and directional cloning. It is an essential reagent for genetic engineering workflows, including gene cloning, plasmid construction, and genomic mapping. Researchers and production facilities in the pharmaceutical, diagnostic, and synthetic biology industries rely on its consistent activity and specificity.

Application

• Recombinant DNA Technology: For the precise cleavage of DNA at specific sites to facilitate gene cloning and plasmid vector preparation.
• Molecular Diagnostics: Used in restriction fragment length polymorphism (RFLP) analysis and other diagnostic assays for genetic screening.
• Genomic Library Construction: Essential for digesting genomic DNA into manageable fragments for sequencing and library generation.
• Synthetic Biology: A key tool in the assembly of genetic circuits and synthetic pathways via standard assembly methods like Golden Gate cloning.
• Biopharmaceutical Production: Employed in the development and quality control of plasmid DNA used in gene therapy and vaccine production.
• Academic & Industrial Research: A fundamental reagent in laboratories conducting genetic research, from basic science to applied biotechnology.

Basic Information

Product Name	Xho I
CAS No.	81295-43-4
Molecular Formula	N/A (Protein/Enzyme)
Molecular Weight	Contact for details
Synonyms	Endodeoxyribonuclease XhoI; XhoI Restriction Enzyme; XhoI Endonuclease; Restriction Enzyme Xho I; XhoI (from Xanthomonas holcicola); EC 3.1.21.4
EINECS	Contact for details

Quality Control

Our Xho I is produced under stringent quality management systems to ensure batch-to-batch consistency and optimal performance. Each lot undergoes rigorous testing for specific activity, purity, and the absence of contaminating nucleases (DNase, RNase) and phosphatases. Certificates of Analysis (COA) detailing activity units, storage buffer composition, and quality specifications are provided with every shipment to support your research and compliance needs.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C ± 5°C in a non-frost-free freezer. Avoid repeated freeze-thaw cycles. The enzyme is supplied in a stabilized storage buffer containing glycerol. Always handle on ice when in use.

Specification

Item	Specification
Appearance	Clear, colorless solution
Identification (Activity)	Cleaves λ DNA at the specific Xho I recognition site
Specific Activity	≥ 20,000 units/mg protein
Purity (SDS-PAGE)	≥ 95%
Contaminating Nuclease Activity	Not detectable
Storage Buffer	10 mM Tris-HCl, 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, pH 7.4

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.