Description

Taq I is a highly specific restriction endonuclease isolated from *Thermus aquaticus*. This enzyme is critical for molecular biology applications requiring precise DNA cleavage at its recognition sequence. It is an essential reagent for genetic engineering, cloning workflows, and genomic analysis. Researchers and production facilities in biotechnology, pharmaceuticals, and academic institutions rely on its consistent activity and purity for reproducible results.

Application

• Molecular Cloning: Essential for DNA fragment preparation and vector linearization in recombinant DNA technology.
• Restriction Fragment Length Polymorphism (RFLP) Analysis: Used for genetic fingerprinting and mutation detection.
• Genomic DNA Mapping: Facilitates the construction of physical maps of genomes.
• Southern Blotting: Key for DNA digestion prior to hybridization and analysis.
• DNA Sequencing Library Preparation: Assists in the fragmentation and preparation of DNA for next-generation sequencing.
• Diagnostic Assay Development: Utilized in the design of tests for genetic disorders and pathogen detection.

Basic Information

Product Name	Taq I
CAS No.	81295-40-1
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Endodeoxyribonuclease Taq I; TaqI Restriction Enzyme; Restriction Endonuclease Taq I; Tsp451; EC 3.1.21.4; Deoxyribonuclease (Site-Specific) Taq I; *Thermus aquaticus* YT-1 restriction endonuclease TaqI
EINECS	Contact for details

Quality Control

Our Taq I is produced under stringent quality management systems. Each lot undergoes rigorous testing for specific activity, purity, and the absence of contaminating nucleases (e.g., exonucleases, non-specific endonucleases) to ensure optimal performance in sensitive applications. Certificates of Analysis (COA) detailing lot-specific data are available upon request.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C ± 5°C in a non-frost-free freezer. Avoid repeated freeze-thaw cycles. The enzyme is supplied in a stabilized storage buffer.

Specification

Item	Specification
Appearance	Clear, colorless solution
Identification (Activity)	Cleaves T/CGA specifically
Specific Activity	≥ 10,000 units/mg protein
Purity (SDS-PAGE)	≥ 95%
Contaminating Nuclease Activity	Not detected
Storage Buffer	10 mM Tris-HCl, 100 mM KCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, pH 7.4

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.