Description

Restriction Endonuclease Mnl I is a type II restriction enzyme that recognizes the specific DNA sequence 5'-CCTC(N)7-3' and cleaves within this recognition site. This product is critical for molecular biology research, enabling precise DNA manipulation and analysis. It is an essential tool for scientists and technicians in biotechnology, pharmaceutical development, and academic research laboratories working on genetic engineering, cloning, and genomic mapping.

Application

• Molecular Cloning: Used for the digestion of DNA fragments prior to ligation and vector construction.
• Restriction Fragment Length Polymorphism (RFLP) Analysis: Employed in genetic fingerprinting and mutation detection studies.
• Genomic DNA Mapping: Facilitates the physical mapping of genomes by generating specific fragment patterns.
• DNA Sequencing Library Preparation: Aids in the preparation of DNA samples for next-generation sequencing platforms.
• Recombinant DNA Technology: Essential for cutting DNA at specific sites to insert or remove genetic material.
• Diagnostic Assay Development: Used in the design and validation of molecular diagnostic tests for genetic disorders.

Basic Information

Product Name	Restriction Endonuclease Mnl I
CAS No.	81295-30-9
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Mnl I Restriction Enzyme; Endodeoxyribonuclease Mnl I; MnlI; Type II Restriction Enzyme Mnl I; Deoxyribonuclease Mnl I; Restriction Enzyme from Moraxella nonliquefaciens; EC 3.1.21.4
EINECS	Contact for details

Quality Control

Our Restriction Endonuclease Mnl I is produced under stringent quality management systems. Each batch undergoes rigorous testing for activity, specificity, and purity, ensuring it is free from contaminating nucleases and phosphatases. Certificates of Analysis (COA) detailing activity units, storage buffer, and purity assessments are available upon request.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C ± 5°C in a non-frost-free freezer. Avoid repeated freeze-thaw cycles. The enzyme is supplied in a stabilized storage buffer containing glycerol.

Specification

Item	Specification
Appearance	Clear, colorless solution
Activity (Units/µl)	≥ 10
Purity (SDS-PAGE)	≥ 95%
Specificity	Cleaves 5'-CCTC(N)7-3'
Contaminating Activities	Devoid of detectable exo- and endonuclease, and phosphatase activities
Storage Buffer	10 mM Tris-HCl, 100 mM KCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol (v/v), pH 7.4 at 25°C

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.