Description

Hind Iii CAS NO 81295-22-9 is a high-purity restriction endonuclease enzyme, a critical tool for precise DNA manipulation in molecular biology research and biotechnological applications. This enzyme is valued for its specific recognition and cleavage sequence, enabling accurate genetic analysis, cloning, and sequencing workflows. It is an essential reagent for pharmaceutical R&D, academic research institutions, and diagnostic kit manufacturers requiring reliable and reproducible enzymatic activity.

Application

• Molecular Cloning: Essential for the precise insertion of DNA fragments into plasmid vectors.
• Genetic Engineering: Used in the construction of recombinant DNA for protein expression and gene function studies.
• DNA Mapping & Analysis: Critical for restriction fragment length polymorphism (RFLP) analysis and genomic mapping.
• Diagnostic Assay Development: Employed in the design and production of diagnostic tests that rely on specific DNA sequence detection.
• Sequencing Library Preparation: Aids in the fragmentation and preparation of DNA samples for next-generation sequencing (NGS).
• Quality Control in Biomanufacturing: Used to verify plasmid identity and integrity in upstream bioprocessing.

Basic Information

Product Name	Hind Iii
CAS No.	81295-22-9
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	HindIII; Endodeoxyribonuclease HindIII; Type II Site-Specific Deoxyribonuclease HindIII; Restriction Enzyme HindIII; EC 3.1.21.4; Deoxyribonuclease (Site-Specific) HindIII
EINECS	Contact for details

Quality Control

Our Hind Iii is produced and tested under stringent quality management systems to ensure batch-to-batch consistency and optimal enzymatic activity. Each lot is rigorously assessed for specificity, absence of non-specific nucleases, and unit activity. A comprehensive Certificate of Analysis (COA) detailing purity, activity, and stability is provided with every shipment to support your quality and regulatory requirements.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C ± 5°C in a non-frost-free freezer. Avoid repeated freeze-thaw cycles. Use appropriate personal protective equipment when handling.

Specification

Item	Specification
Appearance	Clear colorless solution
Identification (Activity)	Cleaves λ DNA at specific recognition sites
Assay (Unit Activity)	≥ 10,000 units/mL
Purity (SDS-PAGE)	≥ 90% single band
Non-Specific Nuclease Activity	Not detected
RNase Activity	Not detected
Storage Buffer	10 mM Tris-HCl, pH 7.4, 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 200 µg/mL BSA, 50% Glycerol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.