Description

N6-Benzoyl-3'-o-T-Butyldimethylsilyl-5'-o-(4,4'-Dimethoxytrityl)-Adenosine is a highly specialized, protected nucleoside derivative that serves as a critical building block in the synthesis of oligonucleotides. Its value lies in providing precise control over the chemical structure and sequence of synthetic DNA and RNA strands, which is fundamental for advanced life science research and therapeutic development. This compound is essential for researchers and manufacturers in the fields of antisense therapy, siRNA development, diagnostic probe synthesis, and next-generation sequencing technologies.

Application

• Solid-Phase Oligonucleotide Synthesis (SPOS): A key phosphoramidite precursor for the automated synthesis of DNA and RNA sequences.
• Antisense Oligonucleotide (ASO) Manufacturing: Used in the production of therapeutic oligonucleotides designed to modulate gene expression.
• siRNA and miRNA Synthesis: Critical for constructing short interfering RNA and microRNA strands for gene silencing research and therapeutics.
• Diagnostic Probe Development: Enables the creation of labeled probes for PCR, FISH, and microarray applications.
• Research Scale Synthesis: Supports academic and pharmaceutical R&D for novel nucleic acid-based compounds.
• Synthesis of Modified Nucleosides: Serves as an intermediate for introducing specific modifications into oligonucleotide backbones.

Basic Information

Product Name	N6-Benzoyl-3'-o-T-Butyldimethylsilyl-5'-o-(4,4'-Dimethoxytrityl)-Adenosine
CAS No.	81256-88-4
Molecular Formula	C44H49N5O7Si
Molecular Weight	788.00 g/mol
Synonyms	5'-O-(4,4'-Dimethoxytrityl)-N6-benzoyl-2'-deoxyadenosine 3'-(2-methylpropyl)dimethylsilyl ether; DMTr-dA(Bz)-TBDMS; N6-Benzoyl-5'-O-(4,4'-dimethoxytrityl)-2'-deoxyadenosine 3'-O-(tert-butyldimethylsilyl) ether; N-Bz-5'-DMTr-dA-3'-TBDMS; Protected Deoxyadenosine Derivative; dA(Bz) Phosphoramidite Precursor
EINECS	Contact for details

Quality Control

This high-purity building block is manufactured under controlled environments to ensure batch-to-batch consistency critical for oligonucleotide synthesis. Quality is verified through a comprehensive suite of analytical techniques including HPLC, NMR, and mass spectrometry. A detailed Certificate of Analysis (COA) is provided with each batch, documenting purity, identity, and moisture content to support your regulatory and research requirements.

Storage

Preserve in a tightly closed container, protected from light. Store in a freezer at -20°C or below under an inert atmosphere (e.g., argon or nitrogen) due to its hygroscopic and moisture-sensitive nature. Allow the sealed container to equilibrate to room temperature before opening to prevent condensation and moisture ingress.

Specification

Item	Specification
Appearance	White to off-white powder or solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.