Description

4-Hydroxymidazolam B-D-Glucuronide is a key metabolite of the sedative-hypnotic agent midazolam, formed via glucuronidation. This compound is of critical importance for pharmaceutical research and development, particularly in the study of drug metabolism, pharmacokinetics, and bioanalytical method validation. It is an essential reference standard and analytical tool for laboratories in the pharmaceutical, forensic toxicology, and clinical research sectors requiring precise quantification and characterization of midazolam and its derivatives.

Application

• Pharmaceutical Reference Standard: Primary use as a certified reference material (CRM) for the quantitative analysis of midazolam metabolites in biological matrices.
• Drug Metabolism & Pharmacokinetics (DMPK) Studies: Critical for in vitro and in vivo studies to understand the metabolic pathways, clearance, and half-life of midazolam.
• Forensic & Clinical Toxicology: Used to calibrate equipment (LC-MS/MS, HPLC) for accurate detection and quantification in post-mortem or compliance testing.
• Bioanalytical Method Development & Validation: Serves as a key analyte for developing robust, sensitive, and specific assays for regulatory submissions.
• Impurity Profiling: Employed in the quality control of midazolam drug substances and products to identify and quantify related substances.
• Enzyme Activity Assays: Used as a substrate to study the activity of UDP-glucuronosyltransferase (UGT) enzymes.

Basic Information

Product Name	4-Hydroxymidazolam B-D-Glucuronide
CAS No.	81256-82-8
Molecular Formula	C30H32ClN5O8
Molecular Weight	626.06 g/mol
Synonyms	1H-Imidazol-1-ium, 4-[7-chloro-5-(2-fluorophenyl)-1,3-dihydro-1-methyl-2H-1,4-benzodiazepin-2-yl]-2-methyl-, (2S,3S,4S,5R,6S)-3,4,5-trihydroxy-6-(hydroxymethyl)tetrahydro-2H-pyran-2-carboxylate; 1'-Hydroxymidazolam Glucuronide; α-Hydroxymidazolam Glucuronide; 4-Hydroxymidazolam Glucuronide; Midazolam Metabolite Glucuronide; 1-Hydroxymidazolam β-D-Glucuronide; 1-OH-Midazolam Glucuronide
EINECS	Contact for details

Quality Control

Our 4-Hydroxymidazolam B-D-Glucuronide is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation (IR, MS), to ensure it meets the high standards required for research and analytical applications. A comprehensive Certificate of Analysis (COA) detailing batch-specific results is provided with every shipment to guarantee traceability, consistency, and reliability for your critical work.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Residual Solvents (GC)	Complies with ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.