Description

1'-Hydroxymidazolam--D-Glucuronide is a key glucuronide metabolite of the sedative-hypnotic agent midazolam. This compound is of critical importance in pharmaceutical research and development for studying drug metabolism, pharmacokinetics, and bioanalytical method validation. It is primarily required by pharmaceutical companies, contract research organizations (CROs), and academic institutions engaged in drug discovery and forensic toxicology.

Application

• Pharmacokinetic and Metabolism Studies: Used as a certified reference standard to quantify and characterize the metabolic pathway of midazolam in vivo.
• Bioanalytical Method Development: Serves as a critical calibrant and quality control material in LC-MS/MS and HPLC methods for therapeutic drug monitoring and forensic analysis.
• Pharmaceutical Impurity Profiling: Employed as an impurity reference standard to ensure the purity and safety of midazolam-based drug formulations.
• Clinical Research: Essential for studies investigating drug-drug interactions, particularly those involving CYP3A4 enzyme activity.
• Forensic Toxicology: Acts as a reference compound for the accurate identification and confirmation of midazolam use in forensic casework.
• Regulatory Compliance Testing: Supports testing to meet FDA, EMA, and ICH guidelines for drug metabolism and disposition studies.

Basic Information

Product Name	1'-Hydroxymidazolam--D-Glucuronide
CAS No.	81256-81-7
Molecular Formula	C30H32ClN6O8
Molecular Weight	639.07 g/mol
Synonyms	1'-Hydroxymidazolam Glucuronide; Midazolam 1'-Hydroxy Glucuronide; 1'-OH-Midazolam Glucuronide; 8-Chloro-6-(2-fluorophenyl)-1-methyl-4H-imidazo[1,5-a][1,4]benzodiazepine-3-carboxylic acid, 1-(β-D-glucopyranuronosyl)-; 1'-Hydroxy Midazolam O-β-D-Glucuronide; α-Hydroxymidazolam Glucuronide; Midazolam Metabolite Glucuronide
EINECS	Contact for details

Quality Control

Our 1'-Hydroxymidazolam--D-Glucuronide is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC and LC-MS, to ensure high purity and identity confirmation. Certificates of Analysis (COA) detailing purity, impurities, and analytical methods are provided with every shipment to support your GLP and GMP-compliant research.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C or below. Allow the product to equilibrate to room temperature before opening to minimize moisture condensation. The material should be handled in a controlled environment.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.