Description

5'-O-DMT-2'-TBDMS-Uridine is a key phosphoramidite monomer, a protected nucleoside derivative essential for the solid-phase synthesis of oligonucleotides. Its primary value lies in providing precise control over the assembly of RNA strands, ensuring high coupling efficiency and yield. This compound is critically needed by researchers and manufacturers in the pharmaceutical, biotechnology, and diagnostic industries for applications in antisense therapy, siRNA development, and molecular diagnostics.

Application

• Solid-Phase Oligonucleotide Synthesis: As a critical building block (phosphoramidite) for the automated synthesis of RNA sequences.
• Therapeutic RNA Development: Used in the research and production of siRNA (small interfering RNA), miRNA, and antisense oligonucleotides for targeted therapies.
• Diagnostic Probe Manufacture: Essential for creating labeled RNA probes used in genomic testing, fluorescence in situ hybridization (FISH), and other diagnostic assays.
• Academic & Industrial Research: A fundamental reagent for studying RNA structure, function, and for developing novel RNA-based tools and therapeutics.
• GMP Production: Serves as a starting material or intermediate in the cGMP-compliant manufacturing of oligonucleotide active pharmaceutical ingredients (APIs).

Basic Information

Item	Details
Product Name	5'-O-DMT-2'-TBDMS-Uridine
CAS No.	81246-80-2
Molecular Formula	C35H48N2O8Si
Molecular Weight	652.86 g/mol
Synonyms	5'-O-(4,4'-Dimethoxytrityl)-2'-O-(tert-butyldimethylsilyl)uridine; 2'-O-TBDMS-5'-O-DMT-uridine; Uridine, 5'-O-[bis(4-methoxyphenyl)phenylmethyl]-2'-O-[(1,1-dimethylethyl)dimethylsilyl]-; DMT-2'-TBDMS-Uridine; 5'-DMT-2'-TBDMS-U; RNA Phosphoramidite Precursor; Uridine DMT TBDMS ether
EINECS	Contact for details

Quality Control

Every batch of 5'-O-DMT-2'-TBDMS-Uridine is manufactured under strict quality management systems and undergoes comprehensive analytical testing. We ensure compliance with stringent internal specifications, which are designed to meet the demanding requirements of oligonucleotide synthesis. A detailed Certificate of Analysis (COA) providing purity, identity, and impurity profile data is supplied with each shipment to guarantee reproducible performance and supply chain traceability.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C or as indicated on the label or COA. This material is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability and purity. Allow the sealed container to reach ambient temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms
Identification (IR)	Conforms
Assay (HPLC)	≥ 98.0%
Water Content (KF)	≤ 0.5%
Related Substances (HPLC)	Total impurities ≤ 2.0% Any single impurity ≤ 1.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.