Description

D-Alanine 4-Nitroanilide Hydrochloride is a high-purity chromogenic substrate specifically designed for enzymatic assays and biochemical research. This compound is critical for the detection and quantification of protease activity, particularly for enzymes that cleave at the C-terminus of D-alanine residues. It is an essential reagent for pharmaceutical R&D, diagnostic kit manufacturers, and academic laboratories focused on enzymology and inhibitor screening. The product is supplied as a stable hydrochloride salt to ensure consistent performance and shelf life.

Application

• Protease Activity Assays: Primary substrate for the colorimetric detection and kinetic study of specific proteases, including bacterial D-alanyl-D-alanine carboxypeptidases and transpeptidases.
• Pharmaceutical Research: Used in high-throughput screening (HTS) platforms to identify and characterize novel antibiotic compounds that target bacterial cell wall synthesis enzymes.
• Diagnostic Development: A key component in the formulation of clinical diagnostic kits for measuring specific enzyme levels in research settings.
• Biochemical Research: Serves as a tool substrate in enzymology studies to investigate substrate specificity, inhibition mechanisms, and enzyme kinetics.
• Quality Control in Biotechnology: Employed as a reference standard in the QC testing of enzyme preparations and related bioproducts.

Basic Information

Product Name	D-Alanine 4-Nitroanilide Hydrochloride
CAS No.	81086-57-9
Molecular Formula	C9H12N4O4 • HCl
Molecular Weight	284.68 g/mol
Synonyms	D-Ala-pNA • HCl; H-D-Ala-p-nitroanilide hydrochloride; (R)-2-Amino-N-(4-nitrophenyl)propanamide hydrochloride; D-Alanine-4-nitroanilide hydrochloride; N-(4-Nitrophenyl)-D-alaninamide hydrochloride; 4-Nitroanilide of D-alanine hydrochloride; Substrate for DD-carboxypeptidase
EINECS	Contact for details

Quality Control

Our D-Alanine 4-Nitroanilide Hydrochloride is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, spectroscopic identification, and residual solvent analysis, to ensure it meets the stringent requirements for research and diagnostic applications. A Certificate of Analysis (COA) detailing all test results is provided with every shipment to guarantee traceability and compliance with your specifications.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. The product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent degradation. For long-term storage, consider desiccant in the storage environment.

Specification

Item	Specification
Appearance	Yellow to orange crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.1%
Heavy Metals	≤ 20 ppm
Specific Rotation	Contact for details
Solubility	Soluble in DMSO, DMF; slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.