Description

2'-Deoxyguanosine-5'-o-(1-Thiotriphosphate), Rp-Isomer Sodium Salt is a chemically modified nucleotide analog where a non-bridging oxygen atom in the α-phosphate group is replaced by sulfur, creating a chiral center at phosphorus. This product is the Rp diastereomer, a critical tool for studying enzyme mechanisms and developing molecular probes due to its resistance to hydrolysis. It is essential for researchers and developers in molecular biology, enzymology, and diagnostic assay development, particularly for investigating phosphotransferase reactions and creating nuclease-resistant oligonucleotides.

Application

• Enzymology Studies: Used as a substrate analog to probe the stereochemical course of phosphoryl transfer reactions catalyzed by polymerases, kinases, and other ATP/GTP-utilizing enzymes.
• Molecular Biology Research: Employed in the synthesis of thiophosphate-labeled nucleic acids for "RNase H" mapping, footprinting assays, and studying nucleic acid-protein interactions.
• Diagnostic Assay Development: Serves as a key component in non-radioactive labeling techniques and the development of sensitive detection systems for various pathogens.
• Antisense & Therapeutic Oligonucleotide Research: Incorporated into oligonucleotides to enhance stability against nuclease degradation, a crucial step in developing therapeutic nucleic acids.
• Structural Biology: Used in X-ray crystallography and NMR studies to capture and analyze transient enzymatic states and transition state analogs.
• High-Throughput Screening (HTS): Acts as a substrate in biochemical assays to identify and characterize inhibitors of nucleic acid-metabolizing enzymes for drug discovery.

Basic Information

Product Name	2'-Deoxyguanosine-5'-o-(1-Thiotriphosphate), Rp-Isomer Sodium Salt
CAS No.	80902-29-0
Molecular Formula	C10H14N5O12P3S · xNa
Molecular Weight	Contact for details
Synonyms	Rp-dGTPαS Sodium Salt; 2'-Deoxyguanosine-5'-(α-thio)triphosphate, Rp Isomer; Guanosine 5'-(α-thiotriphosphate), 2'-deoxy-, Rp-isomer; dGTPαS (Rp); Rp-2'-Deoxyguanosine 5'-O-(1-Thiotriphosphate); Sp-1-(2-Deoxy-β-D-erythro-pentofuranosyl)guanine 5'-(trihydrogen diphosphate) P-α-thiophosphate; Thiophosphate analog of dGTP; Deoxyguanosine triphosphate, α-thio-, Rp isomer
EINECS	Contact for details

Quality Control

Our 2'-Deoxyguanosine-5'-o-(1-Thiotriphosphate), Rp-Isomer Sodium Salt is manufactured under strict quality systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and diastereomeric excess (de) assessment, to ensure it meets the high standards required for sensitive biochemical and diagnostic applications. A comprehensive Certificate of Analysis (COA) detailing identity, purity, and specific impurities is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms
Purity (HPLC)	≥ 95%
Diastereomeric Excess (de, Rp)	≥ 98%
Assay (UV)	≥ 90%
Water Content (KF)	≤ 5%
Solubility	Soluble in water, TE buffer

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.