Description

2'-Deoxyguanosine-5'-o-(1-Thiotriphosphate), Rp-Isomer Sodium Salt is a chemically modified nucleotide analog where a non-bridging oxygen atom in the phosphate group is replaced by sulfur, creating a chiral center at phosphorus. This specific Rp-isomer is a critical tool in molecular biology for studying enzyme mechanisms and conferring nuclease resistance to nucleic acids. It is essential for researchers and manufacturers in pharmaceutical development, diagnostic assay production, and advanced biochemical research, particularly in the study of DNA polymerases and kinases.

Application

• Mechanistic Studies of Enzymes: Used as a substrate analog to investigate the stereochemical course of phosphoryl transfer reactions catalyzed by DNA polymerases, kinases, and other phosphotransferases.
• Synthesis of Nuclease-Resistant Oligonucleotides: Incorporated during solid-phase synthesis to produce phosphorothioate-backbone oligonucleotides for antisense therapeutics, siRNA, and diagnostic probes.
• Single-Turnover Kinetic Analysis: Enables precise measurement of enzyme kinetics due to the slower hydrolysis rate of the phosphorothioate linkage compared to the natural phosphate ester.
• X-ray Crystallography: Serves as a heavy-atom derivative for phase determination in structural biology studies of enzyme-substrate complexes.
• ATP-Analog in Biochemical Assays: Acts as a non-hydrolyzable or slowly hydrolyzable analog of dGTP in various enzymatic assays to trap intermediate states.
• Next-Generation Sequencing (NGS) Reagents: A key raw material in the formulation of specialized nucleotide mixes for certain sequencing platforms and technologies.

Basic Information

Product Name	2'-Deoxyguanosine-5'-o-(1-Thiotriphosphate), Rp-Isomer Sodium Salt
CAS No.	80902-28-9
Molecular Formula	C10H14N5O12P3S · xNa
Molecular Weight	Contact for details
Synonyms	2'-Deoxyguanosine-5'-(1-thiotriphosphate), Rp-isomer sodium salt; Rp-dGTPαS sodium salt; Guanosine-5'-(1-thio)-triphosphate, 2'-deoxy-, Rp-isomer sodium salt; (Rp)-2'-Deoxyguanosine 5'-O-(1-thiotriphosphate) sodium salt; Sp-1-Thio-2'-deoxyguanosine-5'-triphosphate (enantiomer); dGTPαS (Rp) sodium salt; Deoxyguanosine triphosphate, 1-thio-, Rp-isomer, sodium salt; ATP analog, dGTP-α-S (Rp)
EINECS	Contact for details

Quality Control

Our production of this high-purity biochemical adheres to stringent quality protocols. Every batch is characterized using advanced analytical techniques to ensure exact stereochemical purity (Rp-isomer), high chemical purity, and optimal performance in enzymatic applications. A comprehensive Certificate of Analysis (COA) is provided with each shipment, detailing specifications for identity, purity, and impurity profiles. We support compliance with relevant quality frameworks for research and in-vitro diagnostic (IVD) manufacturing.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a non-frost-free freezer. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake. For long-term storage, consider under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms
Identification (NMR)	Conforms
Purity (HPLC)	≥ 95% (Rp-isomer)
Isomeric Purity (Rp-isomer)	≥ 98%
Assay (UV)	≥ 90%
Water Content (KF)	≤ 5%
Solubility	Soluble in water, buffer solutions

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.