Description

Methylsulfonylnorleucyl-Glycyl-Arginine-4-Nitroanilide is a specialized synthetic peptide substrate designed for high-precision biochemical research and diagnostic applications. This compound is critical for researchers and manufacturers requiring reliable and consistent performance in enzymatic activity assays, particularly for proteases like thrombin and trypsin. It is primarily utilized by pharmaceutical R&D laboratories, diagnostic kit manufacturers, and academic institutions focused on enzymology and coagulation studies. Its role as a chromogenic substrate makes it indispensable for quantifying enzyme activity through spectrophotometric methods.

Application

• Protease Activity Assays: Primary substrate for the kinetic study and quantification of serine proteases, including thrombin, trypsin, and related enzymes.
• Diagnostic Reagent Manufacturing: A key component in the formulation of chromogenic test kits for clinical diagnostics, such as coagulation factor tests and liver function panels.
• Pharmaceutical Research & Development: Used in high-throughput screening (HTS) to identify and characterize potential protease inhibitors for drug discovery programs.
• Biochemical Research: Essential tool in academic and industrial laboratories for investigating enzyme mechanisms, kinetics, and substrate specificity.
• Quality Control in Biologics: Employed to monitor and ensure the activity and purity of enzyme-based biopharmaceutical products during production.

Basic Information

Product Name	Methylsulfonylnorleucyl-Glycyl-Arginine-4-Nitroanilide
CAS No.	80894-73-1
Molecular Formula	C24H39N9O8S
Molecular Weight	613.69 g/mol
Synonyms	MSNLGR-pNA; Methylsulfonyl-Norleucyl-Glycyl-Arginine p-Nitroanilide; Tosyl-Gly-Pro-Arg-pNA (related analog); Chromogenic Substrate for Thrombin; Thrombin Substrate I; H-D-CHG-But-Arg-pNA; Boc-Val-Pro-Arg-pNA (structural analog); Peptide 4-Nitroanilide Substrate
EINECS	Contact for details

Quality Control

Our Methylsulfonylnorleucyl-Glycyl-Arginine-4-Nitroanilide is manufactured under strict quality management systems to ensure batch-to-batch consistency and reliability for sensitive research applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity verification and spectrophotometric assays for functional activity. Certificates of Analysis (COA) detailing purity, identity, and performance data are provided with every shipment to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below for long-term stability. This product is hygroscopic (moisture-sensitive); allow the sealed vial to equilibrate to room temperature before opening to prevent condensation and moisture uptake. For extended storage, consider desiccant in the storage environment.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Functional Activity (δA/min/μmol)	Contact for details
Solubility	Soluble in DMSO, DMF; slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.