Description

Valyl-Glycyl-Arginine-4-Nitroanilide is a synthetic tripeptide substrate designed for the specific detection and measurement of enzymatic activity. This compound is of critical importance in biochemical research and pharmaceutical development as a chromogenic substrate for serine proteases, particularly those involved in blood coagulation and fibrinolysis pathways. It is primarily needed by research institutions, diagnostic kit manufacturers, and pharmaceutical companies engaged in enzyme kinetics studies, high-throughput screening, and the development of novel therapeutic agents.

Application

• Enzyme Assay Substrate: A key chromogenic substrate for the quantitative determination of thrombin, plasmin, and other trypsin-like serine proteases in biochemical research.
• Diagnostic Reagent Manufacturing: Used in the production of in-vitro diagnostic (IVD) kits for monitoring blood coagulation disorders and fibrinolysis status.
• Pharmaceutical R&D: Employed in high-throughput screening (HTS) platforms to identify and characterize potential protease inhibitors for drug discovery.
• Biochemical Research: Essential for studying enzyme kinetics, specificity, and inhibition mechanisms in academic and industrial laboratories.
• Quality Control in Biologics: Used to assess the presence and activity of specific proteases during the manufacturing and purification of biopharmaceuticals.

Basic Information

Product Name	Valyl-Glycyl-Arginine-4-Nitroanilide
CAS No.	80798-23-8
Molecular Formula	C23H37N9O6
Molecular Weight	535.60 g/mol
Synonyms	Val-Gly-Arg-pNA; VGR-pNA; H-Val-Gly-Arg-pNA; Valylglycylarginine 4-nitroanilide; Thrombin Substrate I (Chromogenic); S-2366 (Chromogenic Substrate); Boc-Val-Pro-Arg-pNA (related); Benzoyl-Phe-Val-Arg-pNA (related); Protease Substrate VGR-pNA
EINECS	Contact for details

Quality Control

Our Valyl-Glycyl-Arginine-4-Nitroanilide is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation via IR and MS, to ensure it meets the high standards required for research and diagnostic applications. Certificates of Analysis (COA) detailing purity, assay, and impurity profiles are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. Due to its hygroscopic nature, allow the sealed vial to equilibrate to room temperature before opening to prevent moisture condensation. For long-term stability, store desiccated.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Assay	95.0% - 105.0%
Solubility	Soluble in DMSO, DMF; slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.